Porin variation among clinical isolates of Neisseria gonorrhoeae over a 10-year period, as determined by Por variable region typing.
Porin variation among clinical isolates of Neisseria gonorrhoeae over a 10-year period, as determined by Por variable region typing.
复制标题
通过 Por 可变区分型确定 10 年来淋病奈瑟菌临床分离株的孔蛋白变异。
DOI：
10.1086/374563
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Bash,MargaretC
中科院分区：
文献类型：
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作者：
McKnew,DurrieL;Lynn,Freyja;Zenilman,JonathanM;Bash,MargaretC
淋球菌孢子蛋白 (Por) 是潜在的疫苗靶标，也是血清型分型的抗原决定簇。两类Por，PIA和PIB，以及每一类中抗原性不同的变体是由编码表面暴露环的porgene可变区(VR)中的序列变异引起的。每类 5 个 VR 的寡核苷酸探针用于与从 10 年收集的菌株中选出的 282 种临床淋球菌分离株进行棋盘杂交。 PIA 菌株 (n=63) 显示出有限的多样性，90% 的菌株具有 4 种菌株中的一种。 PIB 菌株 (n=219) 更加多样化，尽管已鉴定出几种随时间持续存在的常见菌株类型。发现个体 VR 内的变化是有限的。本研究提供了有关在单一地理区域随时间推移传播的菌株中 Por 多样性的信息，说明了新型端口分型方法的实用性，并对疫苗开发具有影响
TheNeisseria gonorrhoeaeporin protein (Por) is a potential vaccine target and is the antigenic determinant for serovar typing. Two classes of Por, PIA and PIB, and antigenically distinct variants within each class result from sequence variations in theporgene variable regions (VRs) encoding surface-exposed loops. Oligonucleotide probes to 5 VRs of each class were used in checkerboard hybridizations to type 282 clinical gonococcal isolates selected from strains collected over the course of 10 years. PIA strains (n=63) showed limitedpordiversity, with 90% having 1 of 4portypes. PIB strains (n=219) were more diverse, although several commonportypes were identified that persisted over time. Variation within individual VRs was found to be limited. The present study provides information about the diversity of Por in strains circulating in a single geographic region over time, illustrates the utility of a novelportyping method, and has implications for vaccine development