Sequencing of the putative DNA helicase-encoding gene of the Bombyx mori nuclear polyhedrosis virus and fine-mapping of a region involved in host range expansion
Sequencing of the putative DNA helicase-encoding gene of the Bombyx mori nuclear polyhedrosis virus and fine-mapping of a region involved in host range expansion
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DOI：
10.1016/s0378-1119(96)00671-3
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发表时间：
1997-04-29
期刊：
影响因子：
3.5
通讯作者：
Maeda, S
中科院分区：
文献类型：
--
作者：
Kamita, SG;Maeda, S
3666核苷酸(NT)开放阅读框(ORF)及其侧翼区(58.1-62.1MAP单位(M.U))的完整序列。家蚕核型多角体病毒(BmNPV)。该ORF编码一个143 623 Da的蛋白质，在解开双链DNA的蛋白质中有7个共有的基序，表明它是一个DNA解旋酶。一个572个碱基的SacI-HindIII片段，BmScH，先前被证明在同源重组后扩大了Autograph a calfornica NPV(AcNPV)的寄主范围[Maeda等人]。(1993)J.Virol.67,6234-6238]，定位于BmNPV dna hel。通过共转染实验，两个相邻的核苷酸(A和T)被定位在BmScH内，这两个核苷酸似乎是扩大AcNPV宿主范围所必需的最小必需序列。这些相邻的核苷酸编码了BmNPV(Asp)和AcNPV(Ser)之间的单一氨基酸差异。
The complete sequence of a 3666-nucleotide (nt) open reading frame (ORF) and its flanking regions (58.1-62.1 map units (m.u.) from Bombyx mori nuclear polyhedrosis virus (BmNPV)) was determined. This ORF, BmNPV dnahel, encoded a predicted protein of 143 623 Da which possessed seven consensus motifs found in proteins which unwind duplex DNAs, indicating that it is a DNA helicase. A 572-bp SacI-HindIII fragment, BmScH, that was previously shown to expand the host range of Autographa californica NPV (AcNPV) following homologous recombination [Maeda et al. (1993) J. Virol. 67, 6234-6238], was localized within BmNPV dnahel. By cotransfection experiments, two adjacent nt (A and T) that appeared to be the minimal essential sequence necessary to expand the host range of AcNPV, were mapped within BmScH. These adjacent nt encoded a single amino acid difference between BmNPV (Asp) and AcNPV (Ser).