Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation
Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation
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DOI：
10.3168/jds.2009-2563
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发表时间：
2010-04-01
影响因子：
3.5
通讯作者：
Gronwald, W.
中科院分区：
文献类型：
--
作者：
Klein, M. S.;Almstetter, M. F.;Gronwald, W.
在过去的几十年里，奶牛的牛奶产量大幅增加。选择较高的产奶量会影响可用营养物质的分配，更多的能量分配给牛奶合成，而较少的能量分配给生育和健康所必需的生理过程。本研究系统地研究了乳汁代谢产物在哺乳期早期和晚期的丰度，重点研究了与能量代谢有关的代谢物。本研究的目的是确定牛奶成分与奶牛新陈代谢状态的关系。为探讨哺乳期对奶牛生理代谢指标的影响，选择2个不同生产性能的品种，采用高分辨核磁共振波谱和气质联用技术进行研究。我们可以可靠地量化44种不同的牛奶代谢物。结果表明，丙酮和β-羟丁酸等生物标志物与个体奶牛在哺乳早期的代谢状态有明显的相关性。基于这些数据，选择能够很好地应对早期哺乳期代谢压力的奶牛应该成为一种选择。
Milk production in dairy cows has dramatically increased over the past few decades. The selection for higher milk yield affects the partitioning of available nutrients, with more energy being allocated to milk synthesis and less to physiological processes essential to fertility and fitness. In this study, the abundance of numerous milk metabolites in early and late lactation was systematically investigated, with an emphasis on metabolites related to energy metabolism. The aim of the study was the identification and correlation of milk constituents to the metabolic status of the cows. To investigate the influence of lactation stage on physiological and metabolic variables, 2 breeds of different productivity were selected for investigation by high-resolution nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. We could reliably quantify 44 different milk metabolites. The results show that biomarkers such as acetone and beta-hydroxybutyrate are clearly correlated to the metabolic status of the individual cows during early lactation. Based on these data, the selection of cows that cope well with the metabolic stress of early lactation should become an option.