WRKY Transcription Factors Actively Respond to Fusarium oxysporum in Lilium regale
WRKY Transcription Factors Actively Respond to Fusarium oxysporum in Lilium regale
复制标题
WRKY 转录因子对百合中的尖孢镰刀菌有积极反应
DOI：
10.1094/phyto-10-20-0480-r
复制
发表时间：
2021-09-01
期刊：
影响因子：
3.2
通讯作者：
Liu, Diqiu
中科院分区：
文献类型：
--
作者：
Li, Shan;Liu, Guanze;Liu, Diqiu
WRKY转录因子形成一个植物特异性的超脂肪家族，对调节植物发育、胁迫反应和激素信号转导具有重要意义。本研究从野生百合中鉴定出多个WRKY基因（LrWRKY1-35）。系统发育分析将这些WRKY基因分为I ~ III类。ii类WRKY转录因子进一步分为IIa、IIb、IIc、IId和IIe五个亚类。此外，基于实时荧光定量PCR的基因表达谱分析显示，WRKY基因在白头草根、茎、叶和花中存在差异表达。此外，WRKY基因的表达受尖孢镰刀菌侵染以及水杨酸、茉莉酸甲酯、乙烯利和过氧化氢处理的影响。此外，LrWRKY1蛋白定位于洋葱表皮细胞的细胞核。从大肠杆菌中纯化的重组LrWRKY1蛋白特异地结合到含有W-box序列的DNA片段上，酵母单杂交实验表明，LrWRKY1可以激活转录。烟草（Nicotiana tabacum）的共表达实验证实LrWRKY1调节LrPR10-5的表达。此外，LrWRKY1在烟草和东方杂交品种“西伯利亚”（易感染尖孢镰刀菌）中的过表达增加了转基因植株对尖孢镰刀菌的抗性。整体。LrWRKY1调控抗性基因LrPR10-5的表达，参与了L. regale对F. azysporinn的防御反应。本研究为王氏乳杆菌WRKY基因的表达和功能特性研究提供了有价值的信息。
The WRKY transcription factors form a plant-specific superfatnily important for regulating plant development, stress responses, and hormone signal transduction. In this study, many WRKY genes (LrWRKY1-35) were identified in Liliurn regale, which is a wild lily species highly resistant to Fusarium wilt. These WRKY genes were divided into three classes (I to III) based on a phylogenetic analysis. The Class-II WRKY transcription factors were further divided into five subclasses (IIa, IIb, IIc, IId, and IIe). Moreover, the gene expression patterns based on a quantitative real-time PCR analysis revealed the WRKY genes were differentially expressed in the L. regale roots, stems, leaves, and flowers. Additionally, the expression of the WRKY genes was affected by an infection by Fusarium oxysporum as well as by salicylic acid, methyl jasmonate, ethephon, and hydrogen peroxide treatments. Moreover, the LrWRKY1 protein was localized to the nucleus of onion epidermal cells. The recombinant LrWRKY1 protein purified from Escherichia coli bound specifically to DNA fragments containing the W-box sequence, and a yeast one-hybrid assay indicated that LrWRKY1 can activate transcription. A co-expression assay in tobacco (Nicotiana tabacum) confirmed LrWRKY1 regulates the expression of LrPR10-5. Furthermore, the overexpression of LrWRKY1 in tobacco and the Oriental hybrid 'Siberia' (susceptible to F. oxysporum) increased the resistance of the transgenic plants to F. arysponmz. Overall. LrWRKY1 regulates the expression of the resistance gene LrPR10-5 and is involved in the defense response of L. regale to F. azysporinn. This study provides valuable information regarding the expression and functional characteristics of L. regale WRKY genes.