Circulating ceruloplasmin is an important source of copper for normal and malignant animal cells.
Circulating ceruloplasmin is an important source of copper for normal and malignant animal cells.
复制标题
循环铜蓝蛋白是正常和恶性动物细胞铜的重要来源。
DOI：
10.1016/0304-4165(81)90044-1
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Linder,MC
中科院分区：
文献类型：
--
作者：
Campbell,CH;Brown,R;Linder,MC
用~（64）Cu和~（67）Cu在体内和体外研究了正常和恶性细胞对铜蓝蛋白和非铜蓝蛋白血浆池中铜的相对摄取。1.大部分的铜静脉注射给正常和荷瘤大鼠在1小时内被删除，进入肝脏的一个很大的部分。个体组织对铜蓝蛋白与铜离子的表观亲和力存在差异，但根据实际吸收的μg计算时，均显示对铜蓝蛋白的偏好。2.来自两种来源的可观量的铜也被肿瘤吸收，培养的埃利希腹水肿瘤细胞显示出快速吸收和对铜蓝蛋白的明显偏好超过非铜蓝蛋白铜，原代大鼠肌肉细胞培养物也是如此。3.铜蓝蛋白也被正常和肿瘤大鼠组织吸收，但不如铜蓝蛋白铜迅速，通过管理纯[3 H]亮氨酸或[125 I]铜蓝蛋白。铜缺乏并没有加速这一过程。4.它的结论是，至少在大鼠中，血浆铜蓝蛋白是铜的正常和恶性细胞的首选血浆来源，并且血浆铜蓝蛋白上的铜比蛋白质部分更迅速地翻转，这一发现与其作为铜转运蛋白的作用一致。
The relative uptake of copper from ceruloplasmin and non-ceruloplasmin plasma pools, by normal and malignant cells, was investigated in vivo and in vitro, using64Cu and67Cu. 1. Most of the copper administered intravenously to normal and tumor-bearing rats was removed within 1 h, a substantial portion entering the liver. There were differences in the apparent avidity of individual tissues for ceruplasmin vs. ionic copper, but when calculated on the basis of actual μg absorbed, all showed a preference for ceruplasmin. 2. Appreciable amounts of copper from either source were also absorbed by the tumors, and cultured Ehrlich ascites tumor cells showed a rapid uptake and marked preference for ceruplasmin over non-ceruplasmin copper, as did primary rat muscle cell cultures. 3. Ceruplasmin protein was also absorbed by normal and neoplastic rat tissues, but less rapidly than ceruplasmin copper, as determined by administration of pure [3H]leucine- or [125I]ceruloplasmin. Copper deficiency did not accelerate this process. 4. It is concluded that, at least in rat, ceruloplasmin is the preferred plasma source of copper for normal and malignant cells, and that the copper on ceruplasmin turns over more rapidly than the protein moiety, a finding consistent with its role as a copper transport protein.
登录
查看更多内容
缺铜大鼠对铜 67 的代谢。
影响因子：
--
作者：
Owen Ca
通讯作者：
Owen Ca
影响因子：
4.8
作者：
M. Somogyi
通讯作者：
M. Somogyi
白蛋白--cu(II)--氨基酸三元复合物的证据。
DOI：
--
发表时间：
1968
期刊：
Canadian Journal of Biochemistry
影响因子：
--
作者：
B. Sarkar;Y. Wigfield
通讯作者：
Y. Wigfield
激素对大鼠血浆中铜蓝蛋白和铜浓度的影响。
影响因子：
--
作者：
Evans Gw;RE Wiederanders
通讯作者：
RE Wiederanders
影响因子：
15.9
作者：
J. Bush;J. Mahoney;H. Markowitz;C. Gubler;G. Cartwright;M. Wintrobe
通讯作者：
M. Wintrobe