Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells
Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells
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DOI：
10.3390/ma12213583
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发表时间：
2019-11-01
期刊：
影响因子：
3.4
通讯作者：
Bacakova, Lucie
中科院分区：
文献类型：
--
作者：
Doubkova, Martina;Nemcakova, Ivana;Bacakova, Lucie
硅石-1是一种纯硅质形式的沸石，与ZSM-5铝硅酸盐类型的沸石不同，它的结构中不含有潜在有害的铝。本文报道了一种沉积在硅-硅（100）衬底上的硅-1薄膜作为一种潜在的抗腐蚀和生物相容性涂层用于骨科植入物的研究。采用四丙基氢氧化铵（TPAOH）、正硅酸四乙酯（TEOS）和二氢氧化钠（diH(2)O）的混合反应，在硅（100）晶片表面原位制备了硅酸-1薄膜。采用x射线光电子能谱、水接触角测量、原子力显微镜和扫描电镜对所得表面的理化性质进行了表征。通过与MG-63细胞系（人骨肉瘤）的相互作用，从细胞粘附、形态、增殖和活力等方面评估生物相容性。合成的硅石-1薄膜由两层（b-和a， b取向晶体）组成，形成了适合细胞生长的微纳米级表面形态的组合。尽管具有疏水性，但硅石-1膜增加了最初粘附的人成骨样MG-63细胞的数量和这些细胞的增殖率。与参考Si（100）衬底相比，硅石-1薄膜也提高了细胞活力。因此，它是一种很有前途的骨科植入物涂层候选者。
Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH(2)O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.