Metabolism-enhanced tumor localization by fluorescence imaging:: in vivo animal studies
Metabolism-enhanced tumor localization by fluorescence imaging:: in vivo animal studies
复制标题
DOI：
10.1364/ol.28.002070
复制
发表时间：
2003-11-01
期刊：
影响因子：
3.6
通讯作者：
Chance, B
中科院分区：
文献类型：
--
作者：
Chen, Y;Zheng, G;Chance, B
我们提出了一种基于分子标记荧光造影剂的高灵敏度近红外光学成像系统，用于非侵入性癌症检测和定位。该频域系统利用漫射光子密度波的类干涉模式，对嵌入在散射介质中的荧光不均匀实现了高的检测灵敏度和定位精度。通过反射探头几何形状和测角重建得到二维定位图。使用新型Cypate-mono-2-deoxy-葡萄糖荧光造影剂对荷瘤小鼠模型进行体内测量，以增强的肿瘤糖酵解为靶点，证明了在组织样介质中检测2厘米深的亚表面肿瘤的可行性，定位精度在2-3毫米以内。(C)2003年美国光学学会。
We present a high-sensitivity near-infrared optical imaging system for noninvasive cancer detection and localization based on molecularly labeled fluorescent contrast agents. This frequency-domain system utilizes the interferencelike pattern of diffuse photon density waves to achieve high detection sensitivity and localization accuracy for the fluorescent heterogeneity embedded inside the scattering media. A two-dimensional localization map is obtained through reflectance probe geometry and goniometric reconstruction. In vivo measurements with a tumor-bearing mouse model by use of the novel Cypate-mono-2-deoxy-glucose fluorescent contrast agent, which targets the enhanced tumor glycolysis, demonstrate the feasibility of detection of a 2-cm-deep subsurface tumor in the tissuelike medium, with a localization accuracy within 2-3 mm. (C) 2003 Optical Society of America.