Boron nutrition of cultured tobacco BY-2 cells. IV. Genes induced under low boron supply
Boron nutrition of cultured tobacco BY-2 cells. IV. Genes induced under low boron supply
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DOI：
10.1093/jxb/erh142
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发表时间：
2004-06-01
影响因子：
6.9
通讯作者：
Matoh, T
中科院分区：
文献类型：
--
作者：
Kobayashi, M;Mutoh, T;Matoh, T
通过对低硼（B）驯化烟草BY-2（Nicotiana tabacum L.）CV.用cDNA差减法筛选在低浓度B条件下筛选的亮黄2）细胞。13个基因被确定，包括早期水杨酸诱导的葡萄糖基转移酶，谷氨酰胺合成酶，谷胱甘肽S-转移酶，和发病相关的蛋白，这可能构成了一个救援系统的氧化损伤。这表明B缺乏可能会对细胞造成细胞氧化还原失衡。13个基因中的两个在亲本细胞中去除B的30分钟内被诱导，表明信号从细胞壁快速转移到细胞质。
Genes whose expression was up-regulated in low boron (B)-acclimated tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells, which had been selected under a low supply of B, were screened by the cDNA differential subtraction method. Thirteen genes were identified, including early salicylate-inducible glucosyltransferase, glutamine synthetase, glutathione S-transferase, and a pathogenesis-related protein, which might constitute a rescue system for oxidative damage. This indicates that B deficiency might impose cellular redox imbalance on the cells. Two of the 13 genes were induced within 30 min of B removal in the parent cells, indicating fast signal transfer from the cell walls to the cytoplasm.