Quantitative metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry.
Quantitative metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry.
复制标题
通过毛细管电泳飞行时间质谱法对生长激素转基因银大麻哈鱼进行定量代谢组分析。
DOI：
10.1007/978-3-030-00138-4_18
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
T. Soga
中科院分区：
文献类型：
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作者：
T. Nakano;H. Shirakawa;G. Yeo;R.H. Devlin;T. Soga
鱼类的生长部分受生长激素（GH）-胰岛素样生长因子（IGF）轴的调节，已知GH转基因鲑鱼显示出显著的生长增加。然而，很少有人知道在体内的全球水平的代谢产物和生长激素转基因脊椎动物的增长增强的机制。本研究通过代谢组学分析检测了过量表达GH的转GH基因银鲑（Oncorhynchus kisutch）的带电代谢物水平。三个年龄组的大小匹配（0岁，大约。60 g）和年龄匹配（1.5岁）的GH转基因（T）和非转基因（NT）野生鲑鱼的肌肉和肝脏中约200种代谢物的水平进行了定量评估。在转基因鱼和非转基因鱼之间发现的最显著的差异是糖酵解代谢产物水平在转基因鱼的肌肉中增加。此外，在转基因鱼肌肉中的一些代谢物水平的增加被发现是通过定量限制增强。然而，在肌肉中观察到的这些影响与在肝脏中观察到的不同。结果表明，GH转基因可以改善碳水化合物的使用，作为与快速生长相关的能量来源。这些影响可能取决于转基因鱼的总可消化能量摄入水平和组织类型。
Growth in fish is regulated in part by the growth hormone (GH)-insulin-like growth factor (IGF) axis, and salmon transgenic for GH are known to show dramatic increased growth. However, little is known concerning the in vivo global levels of metabolites and the mechanism of enhancement of growth in GH transgenic vertebrates. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) overexpressing GH by metabolomic analysis. Triplicate groups of size-matched (0 year-old, approx. 60 g) and age-matched (1.5 years-old) GH transgenic (T) and non-transgenic (NT) wild salmon were quantitatively assessed for levels of approximately 200 metabolites in both muscle and liver. The most notable difference found between T and NT fish was that glycolysis metabolite levels were increased in the muscle of transgenic fish. In addition, an increase in some metabolite levels in the transgenic fish muscle was found to be enhanced by ration-restriction. However, these effects observed in muscle were different from that seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth. These effects are likely to depend on the level of total digestible energy intake and type of tissue in transgenic fish.