Enchytraeid Reproduction TestPLUS: hatching, growth and full life cycle test-an optional multi-endpoint test with Enchytraeus crypticus
Enchytraeid Reproduction TestPLUS: hatching, growth and full life cycle test-an optional multi-endpoint test with Enchytraeus crypticus
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DOI：
10.1007/s10646-015-1445-5
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发表时间：
2015-07-01
期刊：
影响因子：
2.7
通讯作者：
Amorim, Monica J. B.
中科院分区：
文献类型：
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作者：
Bicho, Rita C.;Santos, Fatima C. F.;Amorim, Monica J. B.
无脊椎动物的土壤生态毒性标准测试通常仅限于评估生存和繁殖等终点。不良反应可能发生在其他发育阶段，例如胚胎发育、孵化或生长。Enchytraeus crypticus物种是标准土壤生态毒理学测试中的模式生物，在该测试中评估存活和繁殖。在本研究中，我们优化了试验方法，以纳入其他终点。所提出的测试从同步年龄生物体开始，并且另外包括孵化成功、生长、成熟状态和全生命周期。这使得可以计算茧产量和人口增长率。结果表明，镉具有胚胎毒性，主要作用于胚胎发育阶段和成熟期。此外，全生命周期测试可以区分胚胎形成前后。增加的灵敏度和全生命周期细节水平使其对新型材料（例如纳米材料）具有潜在的用途，其中作用模式和效应目标未知。[图形]。
Soil ecotoxicity standard tests for invertebrates are usually limited to the assessment of endpoints like survival and reproduction. Adverse effects may occur at other developmental stages, e.g. embryo development, hatching or growth. The species Enchytraeus crypticus is a model organism in the standard soil ecotoxicology test, where survival and reproduction are assessed. In the present study we optimized the test method to include additional endpoints. The proposed test started with synchronized age organisms', and included additionally hatching success, growth, maturation status and full life cycle. This allowed for the calculation of cocoon production and population growth rate. Results indicated that Cd is embryotoxic, main effect occurs on the embryo developmental stage and maturity. Further, the full life cycle test can discriminate between pre- and post-embryo formation. The increased sensitivity and full life cycle detail level makes it potentially useful for novel materials e.g. nanomaterials where the mode of action and hence effect target is unknown.[GRAPHICS].