The role of the actornyosin cytoskeleton in coordination of tissue growth during Drosophila oogenesis
The role of the actornyosin cytoskeleton in coordination of tissue growth during Drosophila oogenesis
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DOI：
10.1016/j.cub.2007.06.067
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发表时间：
2007-08-07
期刊：
影响因子：
9.2
通讯作者：
Riechmann, Veit
中科院分区：
文献类型：
--
作者：
Wang, Ying;Riechmann, Veit
果蝇卵室是由覆盖生殖细胞囊的体细胞上皮组成的器官。卵室形成后，生殖细胞快速生长而不分裂，而上皮细胞的表面通过细胞增殖而扩张[1，2]。这两种组织协调生长和形态发生的机制尚不清楚。在这里，我们确定了肌动球蛋白细胞骨架在这一过程中的作用。我们发现，肌球蛋白的活动仅限于上皮细胞的顶端表面，这是面临着不断增长的囊肿。我们证明，上皮细胞在肌球蛋白活性的情况下崩溃，并表明，变形的上皮细胞的力量来自不断增长的囊肿。因此，肌球蛋白活性通过平衡囊肿生长产生的力来维持上皮形状。此外，我们的数据表明，囊肿生长诱导上皮细胞分裂。此外，我们还展示了肌球蛋白活性的顶端限制是如何控制的。肌球蛋白在顶端皮质被局部Rho激酶激活，在基底外侧皮质被PP 1 β 9 C抑制。此外，我们的数据表明，活性肌球蛋白是由Baz/Par-6/aPKC复合物的顶端锚定。
The Drosophila egg chamber is an organ composed of a somatic epithelium that covers a germline cyst. After egg-chamber formation, the germline cells grow rapidly without dividing while the surface of the epithelium expands by cell proliferation [1, 2]. The mechanisms that coordinate growth and morphogenesis of the two tissues are not known. Here we identify a role for the actomyosin cytoskeleton in this process. We show that myosin activity is restricted to the epithelium's apical surface, which is facing the growing cyst. We demonstrate that the epithelium collapses in the absence of myosin activity and show that the force that deforms the epithelium originates from the growing cyst. Thus, myosin activity maintains epithelial shape by balancing the force emanating from cyst growth. Further, our data indicate that cyst growth induces cell division in the epithelium. In addition, we show how apical restriction of myosin activity is controlled. Myosin is activated at the apical cortex by localized Rho kinase and inhibited at the basolateral cortex by PP1 beta 9C. In addition, our data indicate that active myosin is apically anchored by the Baz/Par-6/aPKC complex.