Assessment of new chemical disinfectants for HBV virucidal activity in a cell culture model
Assessment of new chemical disinfectants for HBV virucidal activity in a cell culture model
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DOI：
10.1016/j.jhin.2003.12.031
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发表时间：
2004-04-01
影响因子：
6.9
通讯作者：
Lemarie, C
中科院分区：
文献类型：
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作者：
Payan, C;Pivert, A;Lemarie, C
在细胞培养模型中对几种新型化学消毒剂进行了杀乙型肝炎病毒 (HBV) 病毒活性的处理。用测试的消毒剂处理含有 10(10.4) HBV DNA 拷贝/mL 的 HBV 感染人血浆。然后使用人肝癌细胞系 HepG2、4% 聚乙二醇和 3 mM 丁酸钠对其进行多次稀释，历时三天。对 12 种产品进行了 37 次检测，每种测试产品最多有 3 个浓度和 3 次暴露时间。未经消毒剂的平均病毒滴度为每毫升10（5.18）感染单位。我们的结果表明，所检查的所有四种洗手液产品、三种表面消毒剂中的两种和三种仪器消毒剂中的两种都具有高活性，无论浓度和暴露时间如何，都能将病毒传播时间减少 10(3) - 10(4) 倍。然而，其他产品（例如其中一种表面消毒剂）仅在浓度高于 0.5% 时才有效 15 分钟。同样，皮肤消毒剂、一种器械消毒剂和洗手剂（稀释至 50%）活性较低或无活性（减少 < 10(3) 倍）。这是第一项使用细胞培养模型来评估新型消毒剂针对 HBV 的杀病毒活性的研究。结果表明，大多数 9/12 产品因 HBs 抗原改变 (8/9) 或可能的包膜破坏 (1 /9) 而具有活性。进一步的研究正在进行中，使用该模型来评估其他化学消毒剂（例如过氧乙酸）对乙肝病毒的活性。 (C) 2004 年医院感染协会。由爱思唯尔有限公司出版。保留所有权利。
Several new chemical disinfectants were processed for Hepatitis B virus (HBV) virucidal activity in a cell culture model. A pooled HBV infected human plasma with 10(10.4) HBV DNA copies/mL was treated with the tested disinfectant. It was then subjected, for three days at several dilutions, to cell culture using the human hepatoma cell line, HepG2, with 4% polyethyleneglycol and 3 mM sodium butyrate. Thirty-seven assays were performed on 12 products, with up to 3 concentrations and 3 time exposures for each product tested. The mean viral titre without disinfectant was 10(5.18) infectious units per mL. Our results showed that products all four hand rubs examined, two of the three surface disinfectants and two of the three instrument disinfectants were highly active whatever concentrations and time exposures, reducing viral times by factors of 10(3) - 10(4). However, other products such as one of the surface disinfectants was only active at concentrations above 0.5% for 15 min. Similarly the skin disinfectant, one of the instrument disinfectants and the hand wash agent (diluted to 50%) were less or not active (of < 10(3) fold reduction). This is the first study using a cell culture model to assess virucidal activity against HBV of new disinfectants. It showed that most 9/12 products were active by either HBs antigen alteration (8/9) or probable envelope disruption (1 /9). Further studies are in progress using this model to assess the activity of other chemical disinfectants such as peracetic acid against HBV. (C) 2004 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.