An integrated genetic linkage map of the laboratory rat
An integrated genetic linkage map of the laboratory rat
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DOI：
10.1007/s003359900812
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发表时间：
1998-07-01
期刊：
影响因子：
2.5
通讯作者：
Jacob, HJ
中科院分区：
文献类型：
--
作者：
Brown, DM;Matise, TC;Jacob, HJ
实验室大鼠（Rattus novegicus）是生理学和病理生理学研究的主要模型系统，自1966年以来，超过422，000篇出版物描述了对大鼠的生物学研究（NCBI/Medline）。大鼠正在成为研究特定疾病的越来越重要的遗传模型，并保留其作为药物开发的主要临床前模型系统的作用。大鼠的初始遗传连锁图谱包含1171个遗传标记中的432个（Jacob et al. 1995），这是由于与小鼠种间杂交相比，所用作图杂交（SHR x BN）的多态性率相对较低。虽然大鼠基因组计划继续在连锁图谱上定位更多标记，但截至1997年11月，已绘制了超过3，200个基因座。目前的图谱构建是使用两个不同的杂交（SHRSP x BN和FHH x ACI）而不是最初的作图杂交。因此，需要提供不同地图之间的整合。我们着手开发一个综合图谱，并增加大鼠遗传图谱上标记的数量。可用于该分析的杂交包括原始作图杂交SHR x BN互交F2（448个标记），GH x BN互交（205个标记）、SS/Mcw × BN互交（235个标记）和FHH/Eur × ACI/Hsd互交（276个标记），这也是一个新的作图组合。来自每个杂交的46只动物用该杂交的多态性标记进行基因分型。这些图谱似乎涵盖了大鼠基因组的绝大多数。这些额外标记的可用性应有助于在更多数量的菌株中进行更完整的全基因组扫描，并在感兴趣的特定基因组区域中提供额外标记。
The laboratory rat, Rattus novegicus, is a major model system for physiological and pathophysiological studies, and since 1966 more than 422,000 publications describe biological studies on the rat (NCBI/Medline). The rat is becoming an increasingly important genetic model for the study of specific diseases, as well as retaining its role as a major preclinical model system for pharmaceutical development. The initial genetic Linkage map of the rat contained 432 genetic markers (Jacob et al. 1995) out of 1171 developed due to the relatively low polymorphism rate of the mapping cross used (SHR x BN) when compared to the interspecific crosses in the mouse. While the rat genome project continues to localize additional markers on the linkage map, and as of 11/97 more than 3,200 loci have been mapped. Current map construction is using two different crosses (SHRSP x BN and FHH x ACI) rather than the initial mapping cross. Consequently there is a need to provide integration among the different maps. We set out to develop an integrated map, as well as increase the number of markers on the rat genetic map.The crosses available for this analysis included the original mapping cross SHR x BN reciprocal F2 intercross (448 markers), a GH x BN intercross (205 markers), a SS/Mcw x BN intercross (235 markers), and a FHH/Eur x ACI/Hsd intercross (276 markers), which is also one of the new mapping crosses. Forty-six animals from each cross were genotyped with markers polymorphic for that cross. The maps appear to cover the vast majority of the rat genome. The availability of these additional markers should facilitate more complete whole genome scans in a greater number of strains and provide additional markers in specific genomic regions of interest.