Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases.
Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases.
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广泛表达的激素刺激腺苷酸环化酶亚家族的两个成员。
DOI：
10.1073/pnas.89.20.9809
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发表时间：
1992
影响因子：
11.1
通讯作者：
Iyengar,R
中科院分区：
文献类型：
--
作者：
Premont,RT;Chen,J;Ma,HW;Ponnapalli,M;Iyengar,R
来自大鼠肝脏和肾脏的编码激素和鸟嘌呤核苷酸刺激的腺苷酸环化酶 [ATP 焦磷酸裂解酶（环化），EC 4.6.1.1]（6 型）的 cDNA 已被克隆和表达。该酶受毛喉素、鸟苷 5'-γ-硫代]三磷酸和异丙肾上腺素加 GTP 的刺激，但不受鸟嘌呤核苷酸结合蛋白的 β γ 亚基的刺激。第二种形式（5型）与6型有75%相似，也已被克隆。 5 型和 6 型 cDNA 都具有多种信息。基于 PCR 的 5 型和 6 型酶 mRNA 检测表明两者分布广泛。同源性分析表明鸟嘌呤核苷酸刺激蛋白调节的腺苷酸环化酶至少有四个不同的亚家族。 5 型和 6 型酶定义了哺乳动物腺苷酸环化酶的一个独特亚家族。一种鸟嘌呤核苷酸结合蛋白调节效应子的多样性可能允许细胞表面信号传递的不同调节模式。
cDNA encoding a hormone- and guanine nucleotide-stimulated adenylyl cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] (type 6) from rat liver and kidney has been cloned and expressed. This enzyme is stimulated by forskolin, guanosine 5'-[gamma-thio]triphosphate, and isoproterenol plus GTP but is not stimulated by beta gamma subunits of guanine nucleotide-binding proteins. A second form (type 5), which is 75% similar to type 6, has also been cloned. Both types 5 and 6 cDNAs have multiple messages. PCR-based detection of the mRNA for the type 5 and 6 enzymes indicates that both are widely distributed. Homology analyses indicate at least four distinct subfamilies of guanine nucleotide stimulatory protein-regulated adenylyl cyclases. Types 5 and 6 enzymes define one distinct subfamily of mammalian adenylyl cyclases. Diversity of one guanine nucleotide-binding protein-regulated effector may allow different modes of regulation of cell-surface signal transmission.