Silk-derived oxygen-generating electrospun patches for enhancing tissue regeneration: Investigation of calcium peroxide role and its effects on controlled oxygen delivery
Silk-derived oxygen-generating electrospun patches for enhancing tissue regeneration: Investigation of calcium peroxide role and its effects on controlled oxygen delivery
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DOI：
10.1016/j.mtla.2020.100877
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发表时间：
2020-12-01
期刊：
影响因子：
3.4
通讯作者：
Moeini, Mohammad
中科院分区：
文献类型：
--
作者：
Aleemardani, Mina;Solouk, Atefeh;Moeini, Mohammad
在组织工程支架中使用生氧生物材料（OGBs）是一种克服为组织再生提供足够氧气限制的迷人方法。然而，开发ogb需要更深刻的理解。本研究将不同浓度（0.1、0.5和1% wt）的过氧化钙（CPO）包埋在具有高透氧性的天然聚合物丝素（SF）中，通过静电纺丝的方法制备了SF-CPO贴片。通过FTIR、XRD、WDX、Alizarin Red S和SDS-Page分析，证明了CPO的加入对SF-CPO贴片性能的影响，以及CPO对贴片性能的影响。电纺丝纤维的体外氧释放证实了在大约180-260 mmHg范围内持续和可控的氧释放至少13天。抑菌试验表明，CPO的存在对抗菌性能有积极影响；随着CPO的增加，抑制区直径增大（25 ~ 47 mm）。细胞研究（细胞粘附、DAPI和MTT）也表明氧对细胞活力和增殖有重要的支持作用。总之，这项研究有助于理解SF-CPO组合对制造静电纺丝纤维的影响，并且似乎有潜力成为组织工程应用的有前途的ogb。
The use of oxygen-generating biomaterials (OGBs) in tissue-engineered scaffolds is a fascinating approach to overcome the limitation in providing adequate oxygen for tissue regeneration. However, a more profound understanding is required to develop OGBs. In this study, by encapsulation of calcium peroxide (CPO) with different concentrations (0.1, 0.5 and 1% wt) within the silk fibroin (SF), a natural polymer with the high permeability of oxygen, through electrospinning, the SF-CPO patches were fabricated. Due to the addition of CPO, the diameter of electrospun fibers was increased from 459.77 +/- 97.17 nm to 13.06 +/- 1.79 mu m. FTIR, XRD, WDX, Alizarin Red S and SDS-Page analyses were used to prove the loading and impact of CPO on the properties of the SF-CPO patches. In vitro oxygen release from electrospun fibers confirmed sustained and controllable oxygen release for at least 13 days in the range of approximately 180-260 mmHg. Antibacterial assay indicated the positive effects of CPO presence on the antibacterial properties; by an increment in CPO, the diameter of the inhibition zone has enhanced (25 to 47 mm). Cellular studies (cell adhesion, DAPI and MTT) also illustrated the significant support of oxygen on cell viability and proliferation. Altogether, this study contributed to understanding the impact of SF-CPO combination on fabricating electrospun fibers and seems to have the potential to become promising OGBs for tissue engineering applications.