Bone-Marrow-Derived Mesenchymal Stem Cell Transplantation Enhances Closing Pressure and Leak Point Pressure in a Female Urinary Incontinence Rat Model
Bone-Marrow-Derived Mesenchymal Stem Cell Transplantation Enhances Closing Pressure and Leak Point Pressure in a Female Urinary Incontinence Rat Model
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DOI：
10.1159/000317322
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Ahn, Youngkeun
中科院分区：
文献类型：
--
作者：
Kim, Sun-Ouck;Na, Hee Sam;Ahn, Youngkeun
目的：本研究的目的是确定尿道周围注射同种异体间充质干细胞（MSCs）是否可以增加大鼠压力性尿失禁模型的漏尿点压力（LPP）。材料与方法：雌性Sprague-Dawley大鼠（230-240 g，n = 30）分为假手术组（C组）、生理盐水处理组（5组）和MSC处理组（M组）。分离双侧阴部神经，然后在尿道两侧注射生理盐水或MSC。治疗后进行LPP和闭合压力（CP）测试。测定尿道移植部位平滑肌细胞的特异性标志物。结果：S组LPP和CP均显著低于对照组。然而，这些在M组中恢复到对照值（p < 0.05）。C、S和M组的LPP分别为29.1 +/- 2.1、22.0 +/- 2.2和43.1 +/- 3.2 cm H2O。C、S和M组的CP分别为27.1 +/- 3.1、21.1 +/- 3.2和32.1 +/- 2.1 cm H2O。注射的MSC对肌肉特异性标记物染色呈阳性。结论：骨髓间充质干细胞可分化为肌源性细胞，并可能参与受损肌肉组织的修复。版权所有（C）2010 S. Karger AG，巴塞尔
Purpose: The purpose of this study was to determine whether periurethral injection of allogenic mesenchymal stem cells (MSCs) could increase the leak point pressure (LPP) in a rat model of stress urinary incontinence. Materials and Methods: Female Sprague-Dawley rats (230-240 g, n = 30) were divided into 3 groups: sham operation (group C), saline-treated (group 5) and MSC-treated (group M). Bilateral pudendal nerve dissection followed by normal saline or MSC injection on both sides of the urethra was done. LPP and closing pressure (CP) testing was performed after the treatment. The specific markers for smooth muscle cells in the transplantation sites of the urethra were determined. Results: Both the LPP and CP were significantly lower in group S than controls. However, these were restored to the control values in group M (p < 0.05). The LPPs of groups C, S and M were 29.1 +/- 2.1, 22.0 +/- 2.2 and 43.1 +/- 3.2 cm H2O, respectively. The CPs of groups C, S and M were 27.1 +/- 3.1, 21.1 +/- 3.2, and 32.1 +/- 2.1 cm H2O, respectively. The injected MSCs stained positive for muscle-specific markers. Conclusion: This study suggests that MSCs might differentiate into muscle lineage cells and may contribute to the repair of damaged muscle tissue. Copyright (C) 2010 S. Karger AG, Basel