Effects of polyrotaxane structure on polyion complexation with DNA
Effects of polyrotaxane structure on polyion complexation with DNA
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DOI：
10.1016/j.stam.2003.12.014
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发表时间：
2004-05-01
影响因子：
5.5
通讯作者：
Yui, N
中科院分区：
文献类型：
--
作者：
Ooya, T;Yamashita, A;Yui, N
合成了用于DNA络合的新型阳离子聚合物AEC-α/E35-TYR-Zs(AEC-α/E35-TYR-Zs)。在这里，AEC基团被引入到α-环糊精(α-CD)的羟基上，这些羟基被缠绕到以大体积端基(聚轮烷)为端基的聚乙二醇链上。我们比较了AEC-α/E35-TYR-Zs与聚乙烯亚胺(PEI)的DNA络合能力。琼脂糖凝胶位移分析和溴化乙烷置换分析表明，穿线α-CD的数量是提高DNA络合能力的主导因素之一。此外，增加AEC-α/E35-TYR-Z中AEC基团的数量会导致络合更加紧密。(C)2004爱思唯尔有限公司。保留所有权利。
Aminoethylcarbamoyl-polyrotaxanes (AEC-alpha/E35-TYR-Zs) were synthesized as a novel cationic polymer for DNA complexation and characterized in terms of physicochemical properties of polyion complex. Here, AEC groups were introduced to hydroxyl groups of alpha-cyclodextrins (alpha-CDs) that are threaded onto a poly(ethylene glycol) (PEG) chain capped with bulky end-groups (polyrotaxane). We examined the ability of DNA complexation of the AEC-alpha/E35-TYR-Zs compared with polyethylenimine (PEI). Agarose gel shift assay and ethydium bromide (EB) displacement analysis showed that the number of threading alpha-CDs was one of the dominant factors to enhance the ability of DNA complexation. In addition, increasing the number of AEC groups in the AEC-alpha/E35-TYR-Zs led to tighter complexation. (C) 2004 Elsevier Ltd. All rights reserved.