Bradford Assay for Determining Protein Concentration.
Bradford Assay for Determining Protein Concentration.
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DOI：
10.1101/pdb.prot102269
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发表时间：
2020-04-01
影响因子：
--
通讯作者：
Urbatsch, Ina L
中科院分区：
文献类型：
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作者：
Kielkopf, Clara L;Bauer, William;Urbatsch, Ina L
Bradford测定法是一种快速且相当灵敏的测定蛋白质浓度的方法。其基于考马斯亮蓝G-250染料在与溶液中的变性蛋白质结合后最大吸光度从465 nm偏移至595 nm。
The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.