The proliferative response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model.
The proliferative response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model.
复制标题
在犬模型中从合成血管移植物中分离出的平滑肌细胞对血小板衍生生长因子的增殖反应。
DOI：
10.1016/s0741-5214(99)70212-0
复制
发表时间：
1999
影响因子：
4.3
通讯作者：
Graham,LM
中科院分区：
文献类型：
--
作者：
Minion,DJ;vandeKerkhove,MP;Goodman,GR;vanAalst,JA;Absood,A;Fox,PL;Graham,LM
先前关于移植物愈合的研究表明，与自体主动脉段相比，移植物段中血小板衍生生长因子（PDGF）的产生增加。本研究的目的是表征移植平滑肌细胞（SMCs）的增殖反应PDGF. Methods胸腹移植植入比格犬。从移植物和近端和远端动脉中收获SMC。在原代培养中用生长曲线评估基底增殖。然后将对PDGF的增殖反应与[3 H]胸苷摄取研究和细胞计数进行比较。最后，PDGF受体的特点与放射性标记的配体bindingassays.ResultsThe生长曲线显示，移植SMC进入对数生长期2天前比主动脉SMC。用PDGF（10 ng/mL）刺激静止的早期传代移植物SMC导致[3 H]胸苷掺入增加1.7 ± 0.1倍，显著低于近端主动脉（11.8 ± 3.0）和远端主动脉（10.2 ± 1.9; P <0.5）的SMC。同样，移植物SMC细胞数量增加1.1 ± 0.1倍，显著低于近端（2.8 ± 0.5）和远端（2.9 ± 0.8）主动脉SMC的增加（P <0.5）。对静止的第一代细胞的结合研究显示，与近端（419 ± 147 fmol/百万细胞）和远端（387 ± 112 fmol/百万细胞）的平滑肌细胞相比，移植物SMC中可用于结合的PDGF受体较少（185 ± 70 fmol/百万细胞）（P <0.5）。三个groups.ConclusionGraft SMC存在于一个慢性增殖状态，但表现出减少增殖反应PDGF和有较少的受体可用于结合PDGF比主动脉SMC在体外。（J Vasc Surg 1999;29：845-51.）
ObjectivePrevious studies on graft healing have shown increased platelet-derived growth factor (PDGF) production in graft segments versus native aortic segments. The purpose of this study was to characterize the proliferative response of graft smooth muscle cells (SMCs) to PDGF.MethodsThoracoabdominal grafts were implanted in beagles. SMCs were harvested from the graft and the proximal and distal aortas. Basal proliferation was assessed with growth curves in primary culture. The proliferative response to PDGF then was compared with [3H]thymidine uptake studies and cell counts. Finally, PDGF receptors were characterized with radio-labeled ligand binding assays.ResultsThe growth curves showed that the graft SMCs entered log-phase growth 2 days earlier than did the aortic SMCs. Stimulation of quiescent early-passage graft SMCs with PDGF (10 ng/mL) resulted in a 1.7 ± 0.1–fold increase in [3H]thymidine incorporation, which was significantly less than that of the SMCs from both the proximal aorta (11.8 ± 3.0) and the distal aorta (10.2 ± 1.9; P < .5). Similarly, the 1.1 ± 0.1–fold increase in graft SMC cell number was significantly less than the increases for both proximal (2.8 ± 0.5) and distal (2.9 ± 0.8) aortic SMCs (P < .5). Binding studies on quiescent first-passage cells showed fewer PDGF receptors available for binding in the graft SMCs (185 ± 70 fmol/million cells) as compared with both the proximal (419 ± 147 fmol/million cells) and the distal (387 ± 112 fmol/million cells) aortas (P < .5). Binding affinity was similar for the three groups.ConclusionGraft SMCs exist in a chronic proliferative state but exhibit a decreased proliferative response to PDGF and have fewer receptors available for binding PDGF than do aortic SMCs in vitro. (J Vasc Surg 1999;29:845-51.)