Retinoic acid suppression of squamous differentiation in human head-and-neck squamous carcinoma cells.
Retinoic acid suppression of squamous differentiation in human head-and-neck squamous carcinoma cells.
复制标题
视黄酸抑制人头颈鳞状癌细胞的鳞状分化。
DOI：
10.1002/ijc.2910480215
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发表时间：
1991
影响因子：
6.4
通讯作者：
Lotan,R
中科院分区：
文献类型：
--
作者：
Poddar,S;Hong,WK;Thacher,SM;Lotan,R
类维生素A（维生素A类似物）抑制正常和恶性上皮细胞的鳞状分化。本研究研究了头颈部鳞状细胞癌（HNSCC）细胞系1483在缺乏和存在β全反式维甲酸（RA）的情况下发生鳞状分化的能力。在培养过程中，这些细胞的生长伴随着角化细胞反式谷氨酰胺酶、天合蛋白和角蛋白KI的增加，这是3种已知的鳞状细胞分化标志物。在提取内源性类维甲酸的脱脂油血清（DLS）中培养的细胞中检测到这些分化标志物的水平高于在含有类维甲酸的胎牛血清（FBS）中培养的细胞。通过对通透化细胞进行标记和使用特异性单克隆或多克隆抗体对细胞提取物进行免疫印迹，发现I μM RA治疗降低了FBS或DLS中培养的细胞中各种分化标志物的水平。钙离子载体的存在刺激了细胞在质膜下交联蛋白形成包膜的能力，但RA抑制了这种能力。这些结果表明，恶性1483 HNSCC细胞在培养中再现了正常鳞状细胞分化的主要特征，而类风湿关节炎抑制了这种分化，就像在正常角质化上皮细胞中一样。
Retinoids (vitamin A analogues) inhibit the squamous differentiation of normal and malignant epithelial cells. This Study investigated the ability of the head‐and‐neck squamous‐cell carcinoma (HNSCC) cell line 1483 to undergo squamous differentiation in the absence and presence of β‐all‐trans retinoic acid (RA). The growth of these cells in culture is accompanied by an increase in keratinocyte trans‐glutaminase, involucrin and keratin KI, 3 established markers of squamous cell differentiation. Higher levels of these differentiation markers were detected in cells cultured in delipidized serum (DLS), from which endogenous retinoids have been extracted, than in cells cultured in fetal bovine serum (FBS), which contains retinoids. Treatment with I μM RA decreased the levels of the various differentiation markers in cells cultured in either FBS or DLS as revealed by labelling of permeabilized cells and by immunoblotting of cell extracts using specific monoclonal or polyclonal antibodies. The cells' ability to cross‐link proteins to form envelopes under the plasma membrane was stimulated in the presence of calcium ionophore but Inhibited by RA. These results indicate that the malignant 1483 HNSCC cells recapitulate the main characteristics of normal squamous‐cell differentiation in culture and that RA suppresses this differentiation as it does in normal keratinizing epithelial cells.