Photoelectrocatalytic inactivation of E. coli XL-1 blue colonies in water
Photoelectrocatalytic inactivation of E. coli XL-1 blue colonies in water
复制标题
水中大肠杆菌 XL-1 蓝色菌落的光电催化灭活
DOI：
10.1002/jctb.2394
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发表时间：
2010
影响因子：
3.4
通讯作者：
I. Poulios
中科院分区：
文献类型：
--
作者：
N. Philippidis;E. Nikolakaki;S. Sotiropoulos;I. Poulios
背景技术背景：本工作的目的是在钛基片上制备Ti 0 2 P-2 5工作电极（TiO 2 /Ti），并对其进行表征，研究其对大肠杆菌的光电催化活性。coli XL-1 blue（E.大肠杆菌）菌落作为模式致病菌，在新型间歇式光电化学反应器中进行了研究。研究结果：在500 °C退火后，用扫描电子显微镜（SEM）和X射线衍射（XRD）分析了TiO 2 /Ti薄膜电极的表面形貌和晶体结构，并通过微分电容测量计算了其平带电位（Vfb =-0.54Vvs. Ag/AgCl）。光电催化（PEC）对大肠杆菌的消毒实验结果表明：大肠杆菌菌落进行了比较，那些电化学（EC）和光催化（PC）灭活的病原体，并显示出显着的协同效应的情况下，PEC消毒，导致，在+1.0 V vs Ag/AgCl电池电压，以增加100%的表观速率常数，ko，与简单的光催化过程相比。重复使用实验表明，工作电极在重复使用至少15次后仍保持其有效性。结论：光电催化灭活大肠杆菌。在一种新型的光电催化反应器中，对人工光照下的大肠杆菌菌落进行了研究。10 3CFU mL-1的E.大肠杆菌菌落遵循一级动力学，而参数，如半导体的类型和微生物的浓度起着重要的作用，影响反应速率常数。
BACKGROUND: The aim of the present work was the preparation of Ti0 2 P-25 working electrodes on Ti substrates (TiO 2 /Ti), their characterization and the study of their photoelectrocatalytic activity towards the inactivation of E. coli XL-1 blue (E. Coli) colonies, used as model pathogenic bacteria, in a novel batch photoelectrochemical reactor. RESULTS: After annealing of the TiO 2 /Ti specimens at 500 °C, the surface morphology and crystal structure of the TiO 2 film electrodes were examined by scanning electronic microscopy (SEM) and X-ray diffraction (XRD), while from differential capacitance measurements the flat band potential was calculated (V fb = -0.54 V versus Ag/AgCl). The results of photoelectrocatalytic (PEC) experiments concerning the disinfection of E. coli colonies were compared with those of electrochemical (EC) and photocatalytic (PC) inactivation of the pathogen and showed a significant synergy effect in the case of PEC disinfection, leading, at +1.0 V vs Ag/AgCl cell voltage, to a 100% increase of the apparent rate constant, k o , in comparison with the simple photocatalytic process. Reuse experiments showed that the working electrode retains its effectiveness after, at least, 15 times of reuse. CONCLUSIONS: The photoelectrocatalytic inactivation of E. coli colonies has been studied under artificial illumination in a novel photoelectrocatalytic reactor. The inactivation of 10 3 CFU mL -1 E. coli colonies followed first-order kinetics, while parameters such as type of semiconductor and concentration of the microorganisms play an important role affecting the reaction rate constant.