Organic acids from leaves, fruits, and rinds of Garcinia cowa
Organic acids from leaves, fruits, and rinds of Garcinia cowa
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DOI：
10.1021/jf011627j
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发表时间：
2002-06-05
影响因子：
6.1
通讯作者：
Sakariah, KK
中科院分区：
文献类型：
--
作者：
Jena, BS;Jayaprakasha, GK;Sakariah, KK
采用高效液相色谱法测定了藤黄果（G. cowa）鲜叶、果实和干果皮中的有机酸。将新鲜的叶子、果实和干燥的果皮在120℃下在15磅/英寸(2)的压力下用水提取20-30分钟，此外，使用索氏提取器在60℃下用溶剂(丙酮和甲醇)提取干燥的果皮各8小时。将样品注入 HPLC，使用 0.01 M 磷酸和甲醇进行梯度洗脱，流速为 0.7 mL/min，使用 210 nm 处的 UV 检测。主要有机酸是 (-)-羟基柠檬酸，其含量分别为 1.7%、2.3% 和 12.7%，存在于叶子、果实和果皮中。 (-)-羟基柠檬酸内酯以及草酸和柠檬酸少量存在于叶、果实和果皮中。这是关于 G. cowa 有机酸成分的第一份报告。
Organic acids in fresh leaves, fruits, and dried rinds of Garcinia cowa (G. cowa) were determined by high-performance liquid chromatography. Fresh leaves, fruits, and dried rinds were extracted with water at 120 degreesC for 20-30 min under 15 Ibs/in(2) pressure, Also, dried rinds were extracted with solvents (acetone and methanol) using a Soxhlet extractor at 60 degreesC for 8 h each. The samples were injected to HPLC under gradient elution with 0.01 M phosphoric acid and methanol with a flow rate of 0.7 mL/min using UV detection at 210 nm. The major organic acid was found to be (-)-hydroxycitric acid present in leaves, fruits, and rinds to the extent of 1.7, 2.3, and 12.7%, respectively. (-)-Hydroxycitric acid lactone, and oxalic and citric acids are present in leaves, fruits, and rinds in minor quantities. This is the first report on the composition of organic acids from G. cowa.