Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
复制标题
梅花WRKY基因家族成员及其冷应激反应的全基因组分析
DOI：
10.3390/genes10110911
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发表时间：
2019-11-01
期刊：
影响因子：
3.5
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
--
作者：
Bao, Fei;Ding, Anqi;Zhang, Qixiang
梅花是一种具有极高观赏、食用和药用价值的蔷薇科乔木，其分布主要受低温的限制。WRKY转录因子基因在植物的生长发育和逆境应答中起着重要作用。然而，WRKY基因家族尚未在梅中表征。从梅基因组中鉴定出58个PmWRKYs。他们被锚定在八个链接组，并分为三个大组。各组的基因结构和基序组成都相当保守。基因重复的调查表明，9个和7个PmWRKYs串联和节段重复，分别安排。PmWRKY在不同组织中有区别地表达（即，根、茎、叶、花和果实。基于RNA-seq数据选择了17个冷相关候选基因。此外，为了研究PmWRKYs在低温下的功能，分析了人工冷处理下的表达模式。结果表明，12个PmWRKYs基因在茎中的表达量差异显著，5个基因在茎中的表达量变化较小。特别地，PmWRKY 18的表达水平在阿坝处理后上调。此外，17 PmWRKYs的时空表达模式进行了分析，在冬季。这些结果表明，17个PmWRKYs是调控梅抗寒性的潜在转录因子。
Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume.