A cell atlas of the adult Drosophila midgut
A cell atlas of the adult Drosophila midgut
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DOI：
10.1073/pnas.1916820117
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发表时间：
2020-01-21
影响因子：
11.1
通讯作者：
Perrimon, Norbert
中科院分区：
文献类型：
--
作者：
Hung, Ruei-Jiun;Hu, Yanhui;Perrimon, Norbert
对成年果蝇中肠的研究使我们对细胞类型多样性、干细胞再生、组织稳态和细胞命运决定有了更多的了解。单细胞 RNA 测序的进步为识别新的细胞类型和分子特征提供了机会。我们使用单细胞 RNA 测序来表征中肠上皮细胞的转录组，并鉴定了代表肠干细胞、肠母细胞、肠内分泌细胞 (EE) 和肠上皮细胞的 22 个不同簇。这种公正的方法恢复了大多数已知的肠道干细胞/肠母细胞和 EE 标记，突出了数据集的高质量，并带来了对肠道干细胞生物学、细胞类型特异性细胞器特征、新转录因子在祖细胞中的作用和肠道区域变异、5 种额外的 EE 肠道激素、EE 激素表达多样性和 EE 的旁分泌功能的见解。为了促进对这个丰富数据集的挖掘，我们提供了一个基于网络的资源，用于可视化单细胞中的基因表达。总而言之，我们的研究为解决中肠上皮基因的功能提供了全面的资源。
Studies of the adult Drosophila midgut have led to many insights in our understanding of cell-type diversity, stem cell regeneration, tissue homeostasis, and cell fate decision. Advances in single-cell RNA sequencing provide opportunities to identify new cell types and molecular features. We used single-cell RNA sequencing to characterize the transcriptome of midgut epithelial cells and identified 22 distinct clusters representing intestinal stem cells, enteroblasts, enteroendocrine cells (EEs), and enterocytes. This unbiased approach recovered most of the known intestinal stem cells/enteroblast and EE markers, highlighting the high quality of the dataset, and led to insights on intestinal stem cell biology, cell type-specific organelle features, the roles of new transcription factors in progenitors and regional variation along the gut, 5 additional EE gut hormones, EE hormonal expression diversity, and paracrine function of EEs. To facilitate mining of this rich dataset, we provide a web-based resource for visualization of gene expression in single cells. Altogether, our study provides a comprehensive resource for addressing functions of genes in the midgut epithelium.