Shrink-induced sorting using integrated nanoscale magnetic traps
Shrink-induced sorting using integrated nanoscale magnetic traps
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DOI：
10.1063/1.4790191
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发表时间：
2013-02-11
影响因子：
4
通讯作者：
Khine, Michelle
中科院分区：
文献类型：
--
作者：
Nawarathna, Dharmakeerthi;Norouzi, Nazila;Khine, Michelle
我们提出了一种塑料微流控器件，它集成了纳米级的磁陷阱(NSMT)，可以分离出高纯度和高产量的磁珠和非磁珠，并且具有前所未有的富集性。数值模拟表明，局域磁场梯度比以前报道的要高得多。我们展示了0.001%磁珠混合物的>；20000倍浓缩。由于我们在所有测试的流速下都能获得高纯度，这是一种坚固、快速、便携和简单的解决方案，可以从适合护理应用的小体积中分选目标物种。我们使用96孔的NSMT从小样本中提取DNA，用于定量聚合酶链式反应(QPCR)。(C)2013年美国物理研究所。[http://dx.doi.org/10.1063/1.4790191]
We present a plastic microfluidic device with integrated nanoscale magnetic traps (NSMTs) that separates magnetic from non-magnetic beads with high purity and throughput, and unprecedented enrichments. Numerical simulations indicate significantly higher localized magnetic field gradients than previously reported. We demonstrated >20 000-fold enrichment for 0.001% magnetic bead mixtures. Since we achieve high purity at all flow-rates tested, this is a robust, rapid, portable, and simple solution to sort target species from small volumes amenable for point-of-care applications. We used the NSMT in a 96 well format to extract DNA from small sample volumes for quantitative polymerase chain reaction (qPCR). (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4790191]