THE SENDAI VIRUS P-GENE EXPRESSES BOTH AN ESSENTIAL PROTEIN AND AN INHIBITOR OF RNA-SYNTHESIS BY SHUFFLING MODULES VIA MESSENGER-RNA EDITING
THE SENDAI VIRUS P-GENE EXPRESSES BOTH AN ESSENTIAL PROTEIN AND AN INHIBITOR OF RNA-SYNTHESIS BY SHUFFLING MODULES VIA MESSENGER-RNA EDITING
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DOI：
10.1002/j.1460-2075.1991.tb07860.x
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发表时间：
1991-10-01
期刊：
影响因子：
11.4
通讯作者：
KOLAKOFSKY, D
中科院分区：
文献类型：
--
作者：
CURRAN, J;BOECK, R;KOLAKOFSKY, D
仙台病毒的P基因表达多达八种蛋白质，其中两种(V和W)仅通过编辑的mRNAs表达；只有P蛋白参与RNA合成。为了研究其他P基因蛋白的功能，我们开发了一个体内系统，其中基因组复制是由质粒产生的病毒蛋白驱动的。我们发现，P在这一过程中是必不可少的，而V和W不仅不是必需的，它们是抑制的。通过使用不同的P基因缺失和不同的转染量，我们提供了P是一个模块蛋白的证据。N-末端结构域(与V和W共有)与L或聚合酶蛋白结合，而C-末端结构域与核蛋白NP结合。提出了副粘病毒RNA合成的模型，并讨论了持续感染过程中负调控的意义。
The P gene of Sendai virus expresses as many as eight proteins, two of which (V and W) are expressed only from edited mRNAs; only the P protein is known to be involved in RNA synthesis. To examine the functions of the other P gene proteins, we developed an in vivo system in which genome replication is driven by plasmid generated viral proteins. We found that P was essential for this process, whereas V and W were not only non-essential they were inhibitory. By using various P gene deletions and varying the amounts of plasmids transfected, we provide evidence that P is a modular protein. The N-terminal domain (shared with V and W) hinds the L or polymerase protein, whereas the C-terminal domain binds the nucleoprotein NP. A model of paramyxovirus RNA synthesis is presented, and the implications of negative regulation during persistent infection are discussed.