COLORADO POTATO BEETLES (LEPTINOTARSA-DECEMLINEATA) ADAPT TO PROTEINASE-INHIBITORS INDUCED IN POTATO LEAVES BY METHYL JASMONATE
COLORADO POTATO BEETLES (LEPTINOTARSA-DECEMLINEATA) ADAPT TO PROTEINASE-INHIBITORS INDUCED IN POTATO LEAVES BY METHYL JASMONATE
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DOI：
10.1016/0022-1910(95)00073-4
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发表时间：
1995-12-01
影响因子：
2.2
通讯作者：
JONGSMA, MA
中科院分区：
文献类型：
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作者：
BOLTER, CJ;JONGSMA, MA
用气态茉莉酸甲酯（MJ）处理马铃薯植株，以获得诱导水平高的半胱氨酸和天冬氨酸蛋白酶抑制剂的叶子。诱导的木瓜蛋白酶抑制剂活性估计为总蛋白的 4%。其他条件产生的叶子具有低水平和中等水平的该抑制剂。当科罗拉多马铃薯甲虫幼虫在含有低、中和高水平木瓜蛋白酶抑制剂的叶子上饲养时，它们的发育是相似的。然而，在高抑制剂饮食中饲养的昆虫中，一般蛋白酶活性显着降低（42%），而对来自 MJ 处理的叶子的汁液中的诱导抑制剂不敏感的蛋白酶活性增加了两倍。在三种叶子类型上饲养的昆虫的肠道中，针对特定半胱氨酸蛋白酶底物 p-Glu-Phe-Leu-pNA 的活性是相同的。然而，在低抑制剂饮食下饲养的昆虫肠道中，MJ 处理的叶子的汁液抑制了多达 67% 的这种活性，而在 MJ 处理的叶子上饲养的昆虫的肠道提取物中仅抑制了 27% 的活性，表明半胱氨酸蛋白酶活性诱导了 2.5 倍对马铃薯蛋白酶抑制剂不敏感。针对另一种特定半胱氨酸蛋白酶底物 L-Arg-pNA 的活性对 MJ 处理的叶子中的抑制剂不敏感，但与那些在木瓜蛋白酶抑制剂水平较低的植物上饲养的昆虫相比，喂食这些叶子的昆虫肠道对这种蛋白酶活性的诱导是 3.5 倍。这些数据表明，科罗拉多马铃薯甲虫幼虫通过合成不敏感的蛋白酶来补偿长期摄入木瓜蛋白酶抑制剂期间受抑制的肠道蛋白水解活性。
Potato plants were treated with gaseous methyl jasmonate (MJ) to obtain leaves with high induced levels of cysteine and aspartic proteinase inhibitors. Induced papain inhibitor activity was estimated at 4% of total protein, Other conditions produced leaves with low and moderate levels of this inhibitor. Development of Colorado potato beetle larvae was similar when they were reared on leaves containing low, moderate and high levels of papain inhibitor, Nevertheless, general proteinase activity was significantly reduced (42%) in insects reared on the high inhibitor diet, while proteinase activity that was insensitive to induced inhibitors in juice from MJ-treated leaves had increased two-fold. Activities towards the specific cysteine proteinase substrate p-Glu-Phe-Leu-pNA were the same in guts from insects reared on the three leaf types, However, juice from MJ-treated leaves inhibited as much as 67% of this activity in guts of insects reared on the low inhibitor diet compared to only 27% of the activity in gut extracts from insects reared on MJ-treated leaves, indicating a 2.5-fold induction of cysteine proteinase activity insensitive to potato proteinase inhibitors. None of the activities towards another specific cysteine proteinase substrate L-Arg-pNA were sensitive to inhibitors from MJ-treated leaves, but guts of insects fed these leaves had a 3.5-fold induction of this proteinase activity compared to those reared on plants containing low papain inhibitor levels. These data suggest that Colorado potato beetle larvae compensated for inhibited gut proteolytic activity during chronic intake of papain inhibitors by synthesizing insensitive proteinase(s).