The nasopharyngeal microbiota of beef cattle before and after transport to a feedlot.
The nasopharyngeal microbiota of beef cattle before and after transport to a feedlot.
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DOI：
10.1186/s12866-017-0978-6
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发表时间：
2017-03-22
期刊：
影响因子：
4.2
通讯作者：
Alexander TW
中科院分区：
文献类型：
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作者：
Holman DB;Timsit E;Amat S;Abbott DW;Buret AG;Alexander TW
鼻咽（NP）微生物群在牛的健康中起着重要作用，包括丰富多样的微生物群落。鼻咽也是与牛呼吸道疾病（BRD）相关的潜在病原体的利基，BRD是饲养场牛中严重且昂贵的疾病。我们使用了14头来自封闭和无病牛群的小母牛，以评估运输到饲养场的牛的NP微生物群的动态。在运输至饲养场之前（第0天）以及第2、7和14天对牛进行采样。NP微生物群的结构在研究过程中发生了显著变化，最大的变化发生在第0天（运输前）和第2天之间（P < 0.001）。系统发育多样性和丰富度在饲养场放置后增加（第2天; P < 0.05）。巴氏杆菌属、芽孢杆菌属和变形杆菌属在第0天富集，链球菌属和不动杆菌属在第2天富集，双歧杆菌属在第7天富集，支原体属在第14天富集。使用PICRUSt评估NP微生物群的功能潜力，揭示了复制和修复以及翻译途径在第14天样品中相对更丰富。这些差异主要由支原体引起。尽管8头牛在一个或多个采样时间点对BRD相关细菌多杀性巴氏杆菌培养呈阳性，但对溶血性曼氏菌或睡眠嗜组织菌培养均呈阳性。本研究调查了饲养场放置对肉牛NP微生物群的影响，为期14天。在运输到饲养场的两天内，NP微生物群发生了显著变化，系统发育多样性和丰富度都有所增加。这些结果表明，在运输到饲养场后，牛的NP微生物群发生突然转变。这可能对理解为什么牛在饲养场放置后最容易发生BRD具有重要意义。本文的在线版本（doi：10.1186/s12866-017-0978-6）包含补充材料，可供授权用户使用。
The nasopharyngeal (NP) microbiota plays an important role in bovine health, comprising a rich and diverse microbial community. The nasopharynx is also the niche for potentially pathogenic agents which are associated with bovine respiratory disease (BRD), a serious and costly illness in feedlot cattle. We used 14 beef heifers from a closed and disease-free herd to assess the dynamics of the NP microbiota of cattle that are transported to a feedlot. Cattle were sampled prior to transport to the feedlot (day 0) and at days 2, 7, and 14. The structure of the NP microbiota changed significantly over the course of the study, with the largest shift occurring between day 0 (prior to transport) and day 2 (P < 0.001). Phylogenetic diversity and richness increased following feedlot placement (day 2; P < 0.05). The genera Pasteurella, Bacillus, and Proteus were enriched at day 0, Streptococcus and Acinetobacter at day 2, Bifidobacterium at day 7, and Mycoplasma at day 14. The functional potential of the NP microbiota was assessed using PICRUSt, revealing that replication and repair, as well as translation pathways, were more relatively abundant in day 14 samples. These differences were driven mostly by Mycoplasma. Although eight cattle were culture-positive for the BRD-associated bacterium Pasteurella multocida at one or more sampling times, none were culture-positive for Mannheimia haemolytica or Histophilus somni. This study investigated the effect that feedlot placement has on the NP microbiota of beef cattle over a 14-d period. Within two days of transport to the feedlot, the NP microbiota changed significantly, increasing in both phylogenetic diversity and richness. These results demonstrate that there is an abrupt shift in the NP microbiota of cattle after transportation to a feedlot. This may have importance for understanding why cattle are most susceptible to BRD after feedlot placement. The online version of this article (doi:10.1186/s12866-017-0978-6) contains supplementary material, which is available to authorized users.