YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters
YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters
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DOI：
10.1007/s10616-006-9017-4
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发表时间：
2006-11-01
期刊：
影响因子：
2.2
通讯作者：
Iijima, Shinji
中科院分区：
文献类型：
--
作者：
Morshed, Mahboob;Ando, Munetoshi;Iijima, Shinji
鸡溶菌酶在输卵管中高表达。该基因的5'调控区含有抑制转录的负元件。为了评估溶菌酶基因表达调控的分子基础，我们研究了该区域的结合蛋白。序列基序分析表明，在这个调控区存在假定的YY 1结合位点。电泳迁移率变动分析显示YY 1与负性元件特异性结合。染色质免疫沉淀实验表明，YY 1与输卵管细胞中的阴性元件特异性结合，而与红细胞中的阴性元件不结合。电泳迁移率改变试验和染色质免疫沉淀试验表明，YY 1也与卵清蛋白基因启动子的负调控区结合，并显示输卵管特异性表达。Western blot分析表明，YY 1在输卵管中有较高水平的表达，细胞核分离实验表明，YY 1定位于染色体和核基质组分中。这些结果表明，在这些基因的负调控区域中有一些特定的功能与多功能转录因子YY 1有关。
Chicken lysozyme is highly expressed in the oviduct. The 5' regulatory region of this gene contains a negative element that represses transcription. To assess the molecular basis underlying the regulation of lysozyme gene expression, we investigated the binding protein to this region. Sequence motif analysis suggested the existence of putative YY1 binding sites in this regulatory region. Electrophoretic mobility shift assay showed the specific binding of YY1 to the negative element. In addition, chromatin immunoprecipitation assay indicated that YY1 specifically bound to the negative element in oviduct cells but not in erythrocytes. It was suggested by electrophoretic mobility shift assay and chromatin immunoprecipitation assay that YY1 also bound to the negative regulatory region in the promoter of the ovalbumin gene which also shows oviduct-specific expression. Western blot analysis showed that YY1 was expressed in relatively high levels in the oviduct and nucleus fractionation experiments showed that YY1 was localized both in chromosome and nuclear matrix fractions. These results suggest that there are some specific roles in the negative regulatory regions of these genes in relation to the multifunctional transcription factor YY1.