Reconstitution of maturating and regulatory lymphocyte subsets after cord blood and BMT in children
Reconstitution of maturating and regulatory lymphocyte subsets after cord blood and BMT in children
复制标题
DOI：
10.1038/bmt.2012.176
复制
发表时间：
2013-03-01
影响因子：
4.8
通讯作者：
Duval, M.
中科院分区：
文献类型：
--
作者：
Charrier, E.;Cordeiro, P.;Duval, M.
脐带血移植（CBT）的一些临床特征可以通过免疫子集重建的特异性来解释，这些免疫子集的成熟阶段或其对GVHD、针对肿瘤或感染因子的耐受性或免疫反应的影响不同。在这里，我们比较了 CBT 和 BMT 后其中几个子集的免疫重建情况。 CBT 后 B 细胞计数恢复得更快。 CD4(+)和CD8(+)细胞计数的恢复没有差异。几个亚群的频率也没有差异：CD4(+) T 细胞室中的 CD45RO(+) 记忆细胞和 CD45RA(+) 幼稚细胞，B 细胞中的 CD27(+)，自然杀伤 (NK) 细胞中的 CD56(bright)、NKG2A(+) 和 KIR+ 细胞，CD25(+)FOXP3(+) 调节性 T 细胞和不变 NKT 细胞。移植后6个月时CBT后胸腺初始CD31(+)CD45RA(+)CD4(+) T细胞的比例较低，并且在1年时两组仍低于正常水平。 CBT后NK细胞扩增更加持续，双阴性NKG2A(-)KIR(-)低反应性细胞减少，双阳性NKG2A(+)KIR+高反应性NK细胞增多。因此，这些结果表明，改善 CBT 结果的进一步研究应尝试改善胸腺生成并利用持续的 NK 细胞重建。骨髓移植 (2013) 48, 376-382; doi：10.1038/bmt.2012.176； 2012 年 10 月 15 日在线发布
Some clinical characteristics of cord blood transplantation (CBT) might be explained by specificities in the reconstitution of immune subsets differing by their maturation stage or their implication in GVHD, tolerance or immune responses against tumor or infectious agents. Here, we compare the immune reconstitution of several of these subsets after CBT and BMT. B-cell count recovery was faster after CBT. There was no difference in the recovery of CD4(+) and CD8(+) cell counts. There was no difference either in the frequency of several subsets: CD45RO(+) memory, and CD45RA(+) naive cells within the CD4(+) T-cell compartment, CD27(+) among B cells, CD56(bright), NKG2A(+), and KIR+ cells among natural killer (NK) cells, CD25(+)FOXP3(+) regulatory T cells and invariant NKT cells. The proportion of the thymic naive CD31(+)CD45RA(+)CD4(+) T cells was lower after CBT at 6 months post-transplant, and was still below normal at 1 year in both groups. NK-cell expansion was more sustained after CBT, with fewer double-negative NKG2A(-)KIR(-) hyporesponsive cells and more double-positive NKG2A(+) KIR+ hyper-responsive NK cells. These results, therefore, indicate that further research to improve CBT outcome should try to improve thymopoieisis and take advantage of the sustained NK-cell reconstitution. Bone Marrow Transplantation (2013) 48, 376-382; doi: 10.1038/bmt.2012.176; published online 15 October 2012