Ordered and ushered; the assembly and translocation of the adhesive type I and p pili.
Ordered and ushered; the assembly and translocation of the adhesive type I and p pili.
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DOI：
10.3390/biology2030841
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发表时间：
2013-06-26
期刊：
影响因子：
4.2
通讯作者：
Waksman G
中科院分区：
文献类型：
--
作者：
Lillington J;Waksman G
I型和P型皮利是尿路致病性大肠杆菌的分子伴侣引导皮利，其允许细菌粘附于宿主细胞受体。菌毛的形成和分泌是由两个辅助蛋白协调的，一个是伴侣蛋白，它催化菌毛亚基折叠并使它们保持在聚合能力状态，另一个是外膜跨纳米机器，引导器，它将它们的组装编排成菌毛并驱动它们通过膜分泌。在这篇综述中，最近的结构和动力学研究相结合，以检查I型和P皮利组装的机制，因为它是目前已知的。我们还研究了如何菌毛生物发生机制的知识已被利用来设计选择性抑制剂的过程。
Type I and P pili are chaperone-usher pili of uropathogenic Escherichia coli, which allow bacteria to adhere to host cell receptors. Pilus formation and secretion are orchestrated by two accessory proteins, a chaperone, which catalyses pilus subunit folding and maintains them in a polymerization-competent state, and an outer membrane-spanning nanomachine, the usher, which choreographs their assembly into a pilus and drives their secretion through the membrane. In this review, recent structures and kinetic studies are combined to examine the mechanism of type I and P pili assembly, as it is currently known. We also investigate how the knowledge of pilus biogenesis mechanisms has been exploited to design selective inhibitors of the process.