Identification and quantitation of bioactive components from honeycomb (Nidus Vespae)
Identification and quantitation of bioactive components from honeycomb (Nidus Vespae)
复制标题
蜂窝状生物活性成分的鉴定和定量（Nidus Vespae）
DOI：
10.1016/j.foodchem.2019.126052
复制
发表时间：
2020-06-01
期刊：
影响因子：
8.8
通讯作者：
Cao, Wei
中科院分区：
文献类型：
--
作者：
Zhao, Haoan;Zhu, Min;Cao, Wei
蜂巢（Nidus Vespae）作为蜂产品工业中的一种农副食品废弃物，其高价值利用的需求不断增长。本研究致力于通过测定理化参数、总酚和总黄酮含量、体外抗氧化能力和生物活性成分来研究蜂窝的理化性质、化学成分和营养价值。采用超高效液相色谱-高分辨率质谱（UHPLC-HRMS）方法，初步鉴定出蜂窝状氢乙醇提取物（EHB）中的76种生物活性成分，其中主要成分为多酚和脂肪酸，并分别通过LC-MS和GC-MS进一步定量。此外，还进行了抗菌活性测试，验证EHB对革兰氏阴性（G(-)）菌和革兰氏阳性（G+）菌均具有抑制作用，有利于蜂窝的高值化利用。
Honeycomb (Nidus Vespae) as an agri-food waste in bee product industry is in soaring demand for high-value utilization. This study is dedicated to investigate the physicochemical properties, chemical composition, and nutritional value of honeycomb by determination of physicochemical parameters, total phenolic and total flavonoid contents, antioxidant capacity in vitro, and bioactive components. By using ultra high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) method, a total of 76 bioactive components from hydro-ethanolic extracts of honeycomb (EHB) were tentatively identified, where the principal ones are polyphenols and fatty acids, which were further quantified by LC-MS and GC-MS, respectively. Moreover, antimicrobial activities test has been conducted, verifying that EHB can inhibit both Gram-negative (G(-)) bacteria and Gram-positive (G+) bacteria, which is beneficial for the high-value utilization of honeycomb.