Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses
Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses
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DOI：
10.1051/vetres/2009062
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发表时间：
2010-03-01
影响因子：
4.4
通讯作者：
Whelan, Adam O.
中科院分区：
文献类型：
--
作者：
Coad, Michael;Clifford, Derek;Whelan, Adam O.
用于控制牛结核病的主要监测手段是清除结核菌素皮试阳性反应的动物。在这项研究中，我们进行了一项纵向调查的免疫学和诊断结果的重复短间隔皮肤试验牛自然感染牛分枝杆菌。以大约60天的间隔，对马兜铃菌素皮试阳性牛进行最多4次皮内比较宫颈皮试。连续试验后，观察到皮试强度显著逐渐降低。与此相反，干扰素-γ（IFN-γ）反应的幅度不受重复皮肤测试的影响，无论是短暂的时间周围的每个皮肤测试或纵向重复测试。在每次皮肤试验后3天内观察到血液白细胞介素-10（IL-10）产生的显著增强，尽管这种增强反应的幅度在试验后第10天恢复到较低水平。应用一种新的多重测定法同时测量7种细胞因子和趋化因子，还发现皮肤测试导致抗原特异性白细胞介素-1 β（IL-1 β）的显著和进行性降低，同时证实了血液中稳定的IFN-γ和升高的IL-10应答。因此，我们已经证明，在牛自然感染M。对于牛，重复短间隔皮试可导致皮试反应性的逐渐降低，这对于检测具有边际或不确定皮试反应的受感染动物具有潜在的负面后果。脱敏作用与降低的IL-1 β和升高的IL-10应答相关，但重要的是，不影响抗原特异性IFN-γ应答。
The principal surveillance tool used to control bovine tuberculosis in cattle is the removal of animals that provide a positive response to the tuberculin skin-test. In this study we performed a longitudinal investigation of the immunological and diagnostic consequences of repeated short-interval skin-tests in cattle naturally infected with Mycobacterium bovis. Tuberculin skin-test positive cattle were subjected to up to four further intradermal comparative cervical skin-tests at approximately 60-day intervals. A significant progressive reduction in the strength of the skin-test was observed after successive tests. In contrast, the magnitude of interferon-gamma (IFN-gamma) responses was not influenced by repeat skin-testing either transiently around the time of each skin-test or longitudinally following repeated tests. A significant boost in blood interleukin-10 (IL-10) production was observed within 3 days following each skin-test although the magnitude of this boosted response returned to lower levels by day 10 post-test. The application of a novel multiplex assay to simultaneously measure seven cytokines and chemokines also identified that skin-testing resulted in a significant and progressive reduction in antigen specific interleukin-1 beta (IL-1 beta) whilst confirming stable IFN-gamma and elevated IL-10 responses in the blood. Therefore, we have demonstrated that in cattle naturally infected with M. bovis, repeat short-interval skin-testing can lead to a progressive reduction in skin-test responsiveness which has potential negative consequences for the detection of infected animals with marginal or inconclusive skin-test responses. The desensitising effect is associated with decreased IL-1 beta and elevated IL-10 responses, but importantly, does not influence antigen specific IFN-gamma responses.