Australian thraustochytrids: Potential production of dietary long-chain omega-3 oils using crude glycerol
Australian thraustochytrids: Potential production of dietary long-chain omega-3 oils using crude glycerol
复制标题
DOI：
10.1016/j.jff.2015.01.039
复制
发表时间：
2015-12-01
影响因子：
5.6
通讯作者：
Blackburn, Susan I.
中科院分区：
文献类型：
--
作者：
Chang, Kim Jye Lee;Paul, Helen;Blackburn, Susan I.
破囊壶菌可以产生大量的二十二碳六烯酸（DHA，22：6 omega 3）。甘油是从植物油和动物脂肪生产生物柴油的副产品，其供应量越来越大。我们调查了澳大利亚破囊壶菌使用粗甘油作为生产长链（LC，>= C-20）ω 3-油的主要碳源的潜力。分析了粗甘油含量，并研究了8株破囊壶菌的生长动力学。与裂殖壶菌属、破囊壶菌属和Ulkenia spp.相比，橙色壶菌属菌株实现了更高的生物量（20 g/L干细胞重量; DCW）和脂质产率（389 mg/g总脂肪酸; TFA）。粗甘油中的杂质阻碍了破囊壶菌的生长，在4天时最大产量为9 g/L DCW和48% DHA TFA。我们的研究表明，异养破囊壶菌有潜力为不断增长的全球人口提供一种安全，环境可持续的替代来源，用于饲料和食品中的有益健康的LC omega-3油。(C)2015爱思唯尔有限公司版权所有。
Thraustochytrids can produce high amounts of docosahexaenoic acid (DHA, 22:6 omega 3). Glycerol, a by-product of biodiesel production from vegetable oil and animal fats, is becoming increasingly available. We investigated the potential of Australian thraustochytrids to use crude glycerol as the main carbon source for production of long-chain (LC, >= C-20) omega 3-oils. Crude glycerol content was analysed, and the growth kinetics of eight thraustochytrid strains was examined with crude glycerol. Aurantiochytrium sp. strains achieved higher biomass (20 g/L dry cell weight; DCW) and lipid yield (389 mg/g total fatty acids; TFA) compared to Schizochytriurn, Thraustochytriurn and Ulkenia spp. Impurities in the crude glycerol hindered growth of thraustochytrids, with maximum yield of 9 g/L DCW and 48% DHA TFA at 4-days. Our research shows the potential of heterotrophic thraustochytrids to provide the growing global population with a secure, environmentally sustainable alternative source of health-benefitting LC omega-3 oils for use in feeds and foods. (C) 2015 Elsevier Ltd. All rights reserved.