Intervertebral disk-like biphasic scaffold-demineralized bone matrix cylinder and poly(polycaprolactone triol malate)-for interbody spine fusion.
Intervertebral disk-like biphasic scaffold-demineralized bone matrix cylinder and poly(polycaprolactone triol malate)-for interbody spine fusion.
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DOI：
10.1177/2041731412454420
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发表时间：
2012
影响因子：
8.2
通讯作者：
Li XJ
中科院分区：
文献类型：
--
作者：
Jin L;Wan Y;Shimer AL;Shen FH;Li XJ
椎间融合是一种保存椎间高度和前路融合的固定方法，但自体骨、同种异体骨和金属融合器的融合有其内源性缺陷。本研究的目的是探讨两相支架模型--天然脱钙骨基质圆柱体--可降解生物材料聚己内酯三醇苹果酸酯--能否作为一种生物移植物用于椎间融合。以苹果酸和聚己内酯为原料缩聚合成了聚己内酯三醇苹果酸酯，然后将聚己内酯三醇苹果酸酯同心片材制成兔股骨脱钙骨基质圆柱体。体外培养1d后，将兔软骨细胞负载到三维支架上，植入裸鼠皮下背袋内。在植入12周后，软骨细胞/支架结构大约是支架结构的两倍。X射线和微型计算机断层扫描显示软骨细胞/支架结构最早在4周形成软骨内骨，并显示骨强度随着时间的推移而增加。组织学染色证实了上述观察结果。8周时，脱钙骨基质柱体内已形成板层骨组织。此外，压缩生物力学测试表明，软骨细胞/支架构建物产生的压缩强度明显高于支架组。这些结果表明，在体内环境中，内相聚己内酯三醇苹果酸酯随着时间的推移而降解，并被新骨取代。
Interbody fusion is an established procedure to preserve disk height and anterior fusion, but fusion with autografts, allografts, and metallic cages has its endogenous shortcomings. The objective of this study is to investigate whether a biphasic scaffold model, the native demineralized bone matrix cylinder in conjunction with degradable biomaterial poly(polycaprolactone triol malate), can be employed as a biological graft for interbody fusion. The poly(polycaprolactone triol malate) was synthesized by polycondensing malic acid and polycaprolactone and then the concentric sheet of poly(polycaprolactone triol malate) was fabricated into the demineralized bone matrix cylinder derived from rabbit femurs. Rabbit chondrocytes were loaded onto the three-dimensional constructs with 1-day in vitro culture and implanted into the subcutaneous dorsal pocket of nude mice. The chondrocytes/scaffold constructs are approximately two folds bigger than the scaffold-alone constructs after 12 weeks of implantation. X-ray and micro-computed tomography imaging showed endochondral bone formation in the chondrocytes/scaffold constructs as early as 4 weeks and showed that the bone intensity increased over time. Histological staining confirmed the above observation. By week 8, lamellar bone tissues were formed inside the demineralized bone matrix cylinder. In addition, the compression biomechanical test showed that the chondrocytes/scaffold constructs produced a significant higher compressive strength compared to the scaffold group. These results demonstrated that the inner-phase poly(polycaprolactone triol malate) degraded over time and was replaced by new bone in an in vivo environment.