MRI-guided stereotaxic targeting in pigs based on a stereotaxic localizer box fitted with an isocentric frame and use of SurgiPlan computer-planning software
MRI-guided stereotaxic targeting in pigs based on a stereotaxic localizer box fitted with an isocentric frame and use of SurgiPlan computer-planning software
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DOI：
10.1016/j.jneumeth.2009.06.019
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发表时间：
2009-10-15
影响因子：
3
通讯作者：
Sorensen, Jens-Christian
中科院分区：
文献类型：
--
作者：
Bjarkam, Carsten R.;Cancian, Graziano;Sorensen, Jens-Christian
我们提出了一种立体定位程序，使MRI引导下的等中心立体定位猪。该手术基于Leksell立体定位弓原理和立体定位器盒，该立体定位器盒具有与临床Leksell系统类似的限定立体定位空间的合并基准标记系统（侧板）。可以将获得的MRI导入临床立体定位软件计划系统（Leksell SurgiPlan，Elekta AB，Sweden）中进行3D重建和坐标计算。在MRI之后，侧板被修改的Leksell弓代替，该Leksell弓容纳用于DBS电极的等中心放置的临床标准操纵器，该装置的机械精度在0.3-0.5 mm之间。将立体定位MRI导入立体定位软件计划系统，平均误差为0.4-0.5 mm，最大误差为0.8- 0.5 mm。0.9 mm。在9头猪的体模和插入的颅骨标记上测量的应用精度在所有平面上均在1 mm以内。在两只小型猪的脑内立体定位空间的整个范围内放置10个锰轨迹后发现的脑内应用准确性是均匀随机分布的，在X、Y和Z平面上在0.7 +/- 0.4、0.5 +/- 0.4和0.7 +/- 0.3 mm范围内。注射神经示踪剂在膝下回的三个小型猪和放置封装的基因修饰的细胞在四个小型猪证实了所描述的程序的准确性和功能性，我们的结论是，设计的技术和仪器，使高精度的立体定位程序在猪，可能有利于未来的大型动物神经科学研究，并概述了类似的立体定位方法在其他动物的技术考虑。(C)2009爱思唯尔有限公司版权所有。
We present a stereotaxic procedure enabling MRI-guided isocentric stereotaxy in pigs. The procedure is based on the Leksell stereotaxic arch principle, and a stereotaxic localizer box with an incorporated fiducial marking system (sideplates) defining a stereotaxic space similar to the clinical Leksell system. The obtained MRIs can be imported for 3D-reconstruction and coordinate calculation in the clinical stereotaxic software planning system (Leksell SurgiPlan, Elekta AB, Sweden). After MRI the sideplates are replaced by a modified Leksell arch accommodating clinical standard manipulators for isocentric placement of DBS-electrodes, neural tracers and therapeutics in the calculated target coordinates.The mechanical accuracy of the device was within 0.3-0.5 mm. Stereotaxic MRIs were imported to the stereotaxic software planning system with a mean error of 0.4-0.5 mm and a max error of 0.8-0.9 mm. Application accuracy measured on a phantom and on inserted skull markers in nine pigs was within 1 mm in all planes. The intracerebral application accuracy found after placement of 10 manganese trajectories within the full extent of the intracerebral stereotaxic space in two minipigs was equally randomly distributed and within 0.7 +/- 0.4; 0.5 +/- 0.4: and 0.7 +/- 0.3 mm in the X, Y, and Z plane. Injection of neural tracers in the subgenual gyrus of three minipigs and placement of encapsulated gene-modified cells in four minipigs confirmed the accuracy and functionality of the described procedure.We conclude that the devised technique and instrumentation enable high-precision stereotaxic procedures in pigs that may benefit future large animal neuroscience research and outline the technical considerations for a similar stereotaxic methodology in other animals. (C) 2009 Elsevier B.V. All rights reserved.