Ultrastructural Characteristics of Neuronal Death and White Matter Injury in Mouse Brain Tissues After Intracerebral Hemorrhage: Coexistence of Ferroptosis, Autophagy, and Necrosis.
Ultrastructural Characteristics of Neuronal Death and White Matter Injury in Mouse Brain Tissues After Intracerebral Hemorrhage: Coexistence of Ferroptosis, Autophagy, and Necrosis.
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DOI：
10.3389/fneur.2018.00581
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发表时间：
2018
影响因子：
3.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Li Q;Weiland A;Chen X;Lan X;Han X;Durham F;Liu X;Wan J;Ziai WC;Hanley DF;Wang J
虽然脑出血（ICH）是一种世界性的毁灭性疾病，但其急性期和慢性期超微结构的病理变化却很少被描述。在这项研究中，透射电子显微镜检查ICH引起的病理超微结构。通过纹状体内注射胶原酶在小鼠中诱导ICH。在急性期（3天）、亚急性期（6天）和慢性期（28天）观察病理变化。与假手术动物相比，我们观察到在ICH急性期受损纹状体中的各种类型的细胞死亡，包括坏死、铁凋亡和自噬。急性期可见纹状体内不同程度的轴突变性，晚期可见同侧纹状体和胼胝体内轴突脱髓鞘。此外，吞噬细胞、常驻小胶质细胞和浸润的单核巨噬细胞存在于红细胞和变性神经元周围，并观察到吞噬红细胞和其他碎片。许多突触出现异常或丢失。本研究系统地分析了小鼠脑出血后超微结构的病理变化，为脑出血的病理学研究提供了有价值的信息。
Although intracerebral hemorrhage (ICH) is a devastating disease worldwide, the pathologic changes in ultrastructure during the acute and chronic phases of ICH are poorly described. In this study, transmission electron microscopy was used to examine the ultrastructure of ICH-induced pathology. ICH was induced in mice by an intrastriatal injection of collagenase. Pathologic changes were observed in the acute (3 days), subacute (6 days), and chronic (28 days) phases. Compared with sham animals, we observed various types of cell death in the injured striatum during the acute phase of ICH, including necrosis, ferroptosis, and autophagy. Different degrees of axon degeneration in the striatum were seen in the acute phase, and axonal demyelination was observed in the ipsilateral striatum and corpus callosum at late time points. In addition, phagocytes, resident microglia, and infiltrating monocyte-macrophages were present around red blood cells and degenerating neurons and were observed to engulf red blood cells and other debris. Many synapses appeared abnormal or were lost. This systematic analysis of the pathologic changes in ultrastructure after ICH in mice provides information that will be valuable for future ICH pathology studies.
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影响因子：
64.5
作者：
Dixon SJ;Lemberg KM;Lamprecht MR;Skouta R;Zaitsev EM;Gleason CE;Patel DN;Bauer AJ;Cantley AM;Yang WS;Morrison B 3rd;Stockwell BR
通讯作者：
Stockwell BR
影响因子：
2.9
作者：
Hu S;Xi G;Jin H;He Y;Keep RF;Hua Y
通讯作者：
Hua Y
影响因子：
5.3
作者：
Ducruet, Andrew F.;Zacharia, Brad E.;Hickman, Zachary L.;Grobelny, Bartosz T.;Yeh, Mason L.;Sosunov, Sergey A.;Connolly, E. Sander, Jr.
通讯作者：
Connolly, E. Sander, Jr.
DOI：
10.1016/j.bbi.2016.12.012
发表时间：
2017-03
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Lan X;Han X;Li Q;Li Q;Gao Y;Cheng T;Wan J;Zhu W;Wang J
通讯作者：
Wang J
影响因子：
6.3
作者：
Li, Qian;Wan, Jieru;Wang, Jian
通讯作者：
Wang, Jian