A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster
A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster
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DOI：
10.1177/0748730419895154
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发表时间：
2019-12
影响因子：
3.5
通讯作者：
Manabu Sekiguchi;Kotaro Inoue;Tian Yang;D. Luo;Taishi Yoshii
中科院分区：
文献类型：
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作者：
Manabu Sekiguchi;Kotaro Inoue;Tian Yang;D. Luo;Taishi Yoshii
生理、新陈代谢和行为的日常节律是由中央生物钟精心安排的。在小鼠中，这个时钟由视交叉上核协调，视交叉上核由 20,000 个神经元组成，这使得表征单个神经元具有挑战性。在果蝇中，时钟仅由分布在果蝇大脑中的 150 个时钟神经元控制。在这里，我们描述了一套全面的遗传驱动因素，以促进果蝇时钟神经元的个体表征。我们筛选了从果蝇储备中心获得的 GAL4 系，并鉴定了 63 个在中央时钟神经元亚群中表现出表达的系。此外，我们生成了在时钟神经元子集中（例如 2 个 DN2 神经元和 6 个 LPN 神经元）表现出特异性表达的 split-GAL4 系。与现有的驱动线一起，这些新发现的驱动线是多功能工具，将有助于更好地了解果蝇中央生物钟。
Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.