Enrichment Broth Improved Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Samples from Patients in Intensive Care Units
Enrichment Broth Improved Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Samples from Patients in Intensive Care Units
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DOI：
10.1128/jcm.01406-08
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发表时间：
2009-06-01
影响因子：
9.4
通讯作者：
Debets-Ossenkopp, Yvette J.
中科院分区：
文献类型：
--
作者：
Murk, Jean-Luc A. N.;Heddema, Edou R.;Debets-Ossenkopp, Yvette J.
我们评价了胰蛋白胨大豆肉汤（TSB）用于提高产超广谱β-内酰胺酶（ESBL（+））细菌检测的效果。在固体培养基上接种之前，将咽拭子和直肠拭子在TSB中预富集，可使从我们重症监护室患者中获得的样本中检出的ESBL（+）细菌数量增加一倍。
We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL(+)) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL(+) bacteria detected in samples obtained from patients in our intensive care unit.