REGULATED SPLICING OF ADENOVIRUS TYPE-5 E4-TRANSCRIPTS AND REGULATED CYTOPLASMIC ACCUMULATION OF E4 MESSENGER-RNA
REGULATED SPLICING OF ADENOVIRUS TYPE-5 E4-TRANSCRIPTS AND REGULATED CYTOPLASMIC ACCUMULATION OF E4 MESSENGER-RNA
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DOI：
10.1128/jvi.67.6.3226-3231.1993
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发表时间：
1993-06-01
影响因子：
5.4
通讯作者：
LEPPARD, KN
中科院分区：
文献类型：
--
作者：
DIX, I;LEPPARD, KN
人C型腺病毒的E4基因先前已显示通过差异剪接产生一系列mRNA。在这项研究中，这些mRNA的表达模式在裂解感染进行了检查，并定义了两个不同的时间类。早期类的mRNA与晚期类的mRNA的区别在于，在早期类中，mRNA的3'半中存在一个序列，该序列在晚期类中作为内含子被去除。一个单一的mRNA的晚类被发现表现出强烈的依赖于存在的55 kDa的蛋白质从区域E1 b和开放的阅读框架6蛋白从区域E4的正常细胞质积累。这种mRNA区别于其他晚期表达的E4 mRNA的一个特征是保留了E4 5'端的内含子;因此它可能被宿主细胞识别为不完全剪接并保留在细胞核中。有人提出，E1 b 55-kDa/E4开放阅读框架6蛋白复合物通过克服这种保留机制促进这种mRNA的积累。
The E4 gene of human type C adenoviruses has been shown previously to give rise to an array of mRNAs via differential splicing. In this study, the pattern of expression of these mRNAs during lytic infection was examined, and two distinct temporal classes were defined. mRNAs of the early class were distinguished from those of the late class by the presence, in the early class, of a sequence in the 3' half of the mRNA that was removed as an intron in the late class. A single mRNA of the late class was found to show a strong dependence on the presence of the 55-kDa protein from region E1b and the open reading frame 6 protein from region E4 for its normal cytoplasmic accumulation. One feature of this mRNA that distinguishes it from other E4 mRNAs expressed at late times is the retention within it of an intron from the 5' half of E4; it may therefore be recognized as incompletely spliced by the host cell and retained in the nucleus. It is proposed that the E1b 55-kDa/E4 open reading frame 6 protein complex facilitates accumulation of this mRNA by overcoming this retention mechanism.