Diversity of satellite glia in sympathetic and sensory ganglia.
Diversity of satellite glia in sympathetic and sensory ganglia.
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DOI：
10.1016/j.celrep.2022.110328
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发表时间：
2022-02-01
期刊：
影响因子：
8.8
通讯作者：
Kuruvilla R
中科院分区：
文献类型：
--
作者：
Mapps AA;Thomsen MB;Boehm E;Zhao H;Hattar S;Kuruvilla R
卫星胶质细胞是周围神经系统中交感神经节和感觉神经节中发现的主要胶质细胞类型，并且特异性地接触神经元细胞体。交感神经元和感觉神经元在形态、分子和电生理特性上不同。然而，相关的卫星神经胶质细胞的分子多样性仍然不清楚。在这里，使用单细胞RNA测序分析，我们确定了五个不同的群体的卫星神经胶质细胞的交感神经节和感觉神经节。我们定义了三个共享的卫星神经胶质细胞的人口丰富的免疫反应基因，立即早期基因，离子通道/ECM相互作用，分别。感觉和交感神经特异性卫星胶质细胞的脂质合成和代谢的调节剂差异丰富。感觉神经胶质细胞也特别富含参与谷氨酸周转的基因。此外，卫星神经胶质细胞和许旺细胞可以通过独特的转录特征来区分。这项研究揭示了周围神经系统中卫星胶质细胞的显着异质性。Mapps et.等人使用单细胞转录谱和smFISH显示了交感神经和感觉神经系统中卫星神经胶质细胞的分子多样性，揭示了卫星神经胶质细胞被转录调节以适应神经节特异性功能。
Satellite glia are the major glial type found in sympathetic and sensory ganglia in the peripheral nervous system, and specifically contact neuronal cell bodies. Sympathetic and sensory neurons differ in morphological, molecular, and electrophysiological properties. However, the molecular diversity of the associated satellite glial cells remains unclear. Here, using single cell RNA sequencing analysis, we identify five different populations of satellite glia from sympathetic and sensory ganglia. We define three shared populations of satellite glia enriched in immune-response genes, immediate-early genes, and ion channels/ECM-interactors, respectively. Sensory- and sympathetic-specific satellite glia are differentially enriched for modulators of lipid synthesis and metabolism. Sensory glia are also specifically enriched for genes involved in glutamate turnover. Further, satellite glia and Schwann cells can be distinguished by unique transcriptional signatures. This study reveals remarkable heterogeneity of satellite glia in the peripheral nervous system. Mapps et. al. show the molecular diversity of satellite glial cells in the sympathetic and sensory nervous systems using single-cell transcriptional profiling and smFISH, revealing that satellite glia are transcriptionally tuned to accommodate ganglion-specific functions.