Electron microscopy approach for the visualization of the epithelial and endothelial glycocalyx
Electron microscopy approach for the visualization of the epithelial and endothelial glycocalyx
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DOI：
10.1016/j.morpho.2017.04.001
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发表时间：
2017-06-01
期刊：
影响因子：
--
通讯作者：
Richard, V.
中科院分区：
文献类型：
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作者：
Chevalier, L.;Selim, J.;Richard, V.
这项研究提供了一种通过电子显微镜观察糖萼的方法学方法。糖萼是一个三维网络，主要由与质膜相关的糖脂、糖蛋白和蛋白多糖组成。在不到十年的时间里，上皮性和内皮性糖萼在生理和病理中发挥着重要的作用，引起了人们对其研究的兴趣，特别是在血管功能方面。因此，糖萼的可视化需要可靠的技术，由于其脆弱和动态的组织结构，对电子显微镜采样的不同过程步骤高度敏感，因此其保存仍然具有挑战性。在这项研究中，化学固定是一种很好的替代传统固定的方法。在固定剂中添加硝酸镧增强了在透射电子显微镜明场中糖萼的染色，并通过检测弹性散射电子来改善其可见性，从而提供了化学对比。(C)2017年爱思唯尔·马森公司。版权所有。
This study presents a methodological approach for the visualization of the glycocalyx by electron microscopy. The glycocalyx is a three dimensional network mainly composed of glycolipids, glycoproteins and proteoglycans associated with the plasma membrane. Since less than a decade, the epithelial and endothelial glycocalyx proved to play an important role in physiology and pathology, increasing its research interest especially in vascular functions. Therefore, visualization of the glycocalyx requires reliable techniques and its preservation remains challenging due to its fragile and dynamic organization, which is highly sensitive to the different process steps for electron microscopy sampling. In this study, chemical fixation was performed by perfusion as a good alternative to conventional fixation. Additional lanthanum nitrate in the fixative enhances staining of the glycocalyx in transmission electron microscopy bright field and improves its visualization by detecting the elastic scattered electrons, thus providing a chemical contrast. (C) 2017 Elsevier Masson SAS. All rights reserved.