Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood.
Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood.
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DOI：
10.1186/1743-422x-7-85
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发表时间：
2010-04-30
期刊：
影响因子：
4.8
通讯作者：
Principi N
中科院分区：
文献类型：
--
作者：
Esposito S;Molteni CG;Daleno C;Valzano A;Tagliabue C;Galeone C;Milani G;Fossali E;Marchisio P;Principi N
本研究评价了203名6个月至5岁有呼吸道疾病体征和症状的儿童的父母使用的儿童中鼻甲鼻丛拭子的有效性。从每个孩子身上随机采集两个鼻腔样本：一个由受过训练的儿科医生采集，一个由父母采集。实时聚合酶链反应流感病毒的检出率是相似的，在使用两种方法收集的样本（科恩的kappa = 0.86），作为循环阈值。以儿科医生采集的样本为对照，其敏感性和特异性分别为89.3%（95%置信区间[CI]：77.8-100%）和97.7%（95% CI：95.5-100%），阳性预测值为86.2%（95% CI：73.7-95.1%），阴性预测值为98.2%（95% CI：96.4-100%）。孩子们对父母的收藏品更满意（中位数±标准差，1.59 ± 0.55 vs 3.51 ± 0.36; p < 0.0001）。这些研究结果表明，专门为婴儿和儿童设计的中鼻甲鼻植绒拭子可供父母使用，而不会降低流感病毒的检出率。此外，父母的直接参与显著提高了患者的接受度，从而简化了采集，并表明这种新型拭子设计应考虑用于流行病学调查和疫苗有效性研究。
This study evaluated the efficiency of pediatric mid-turbinate nasal flocked swabs used by parents in 203 children aged 6 months to 5 years with signs and symptoms of respiratory disease. Two nasal samples were collected from each child in a randomised sequence: one by a trained pediatrician and one by a parent. The real-time polymerase chain reaction influenza virus detection rates were similar in the samples collected using the two methods (Cohen's kappa = 0.86), as were the cycle threshold values. In comparison with the pediatrician-collected samples, the sensitivity and specificity of the parental collections were respectively 89.3% (95% confidence interval [CI]: 77.8-100%) and 97.7% (95% CI: 95.5-100%), and the positive and negative predictive values were respectively 86.2% (95% CI: 73.7-95.1%) and 98.2% (95% CI: 96.4-100%). The children were significantly more satisfied with the parental collections (median values ± standard deviation, 1.59 ± 0.55 vs 3.51 ± 0.36; p < 0.0001). These findings show that mid-turbinate nasal flocked swabs specifically designed for infants and children can be used by parents without reducing the influenza virus detection rate. Moreover, the direct involvement of parents significantly increases patient acceptance, thus simplifying collection and suggesting that this novel swab design should be considered for epidemiological surveys and vaccine efficacy studies.