B cell responses in gastric antrum and duodenum following oral inactivated Helicobacter pylori whole cell (HWC) vaccine and LT(R192G) in H pylori seronegative individuals.
B cell responses in gastric antrum and duodenum following oral inactivated Helicobacter pylori whole cell (HWC) vaccine and LT(R192G) in H pylori seronegative individuals.
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DOI：
10.1016/s0264-410x(02)00259-1
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发表时间：
2003-01
期刊：
影响因子：
5.5
通讯作者：
G. Losonsky;K. Kotloff;R. Walker
中科院分区：
文献类型：
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作者：
G. Losonsky;K. Kotloff;R. Walker
为研究幽门螺杆菌（HP）未感染受试者的胃粘膜中是否可引发B细胞特异性应答，5名志愿者摄入3剂HP灭活全细胞（HWC）疫苗和25μg重组不耐热毒素（LTR 192 G）。三名受试者中的两名具有可检测的LTR 192 G和HWC伊加抗体分泌细胞（ASC）胃反应。十二指肠组织中LTR 192 G和HWC的反应是胃窦组织中的5-14倍（分别为P<0.01和P=0.05）。这些结果提供了第一个证据表明，特异性胃B细胞反应可以诱导在HP-非感染的个人口服免疫。
To investigate whether B cell-specific responses could be elicited in the gastric mucosa of Helicobacter pylori (HP) naive subjects, five volunteers ingested three doses of a HP killed whole cell (HWC) vaccine with 25μg of recombinant heat-labile toxin (LTR192G). Two of three subjects had detectable LTR192Gand HWC IgA antibody secreting cell (ASC) gastric responses. LTR192Gand HWC responses in duodenal were 5–14-fold higher than those detected in antral biopsies (P<0.01 and P=0.05, respectively). These results provide the first evidence that specific gastric B cell responses can be induced in HP-non-infected individuals following oral immunization.