Illuminating the role of caspases during Drosophila oogenesis
Illuminating the role of caspases during Drosophila oogenesis
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DOI：
10.1038/sj.cdd.4401892
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发表时间：
2006-11-01
影响因子：
12.4
通讯作者：
Cooley, L.
中科院分区：
文献类型：
--
作者：
Mazzalupo, S.;Cooley, L.
细胞死亡是动物种系发育的一个显着特征。在果蝇中，15 个护理细胞的死亡与每个卵母细胞的发育有关。此外，雌性通过在卵母细胞摄取卵黄之前诱导卵室死亡来应对恶劣的环境条件。为了研究这两种形式的细胞死亡，我们通过表达编码侧翼青色荧光蛋白和黄色荧光蛋白的半胱天冬酶切割位点的转基因来分析种系中的半胱天冬酶活性。当在经历饥饿诱导的细胞凋亡的卵巢中表达时，该构建体是 caspase 活性的准确报告者。然而，在正常卵子发生结束时死亡的护理细胞没有显示出细胞质半胱天冬酶活性的证据。此外，虽然半胱天冬酶抑制剂 p35 或果蝇凋亡蛋白抑制剂 1 的表达阻止了饥饿诱导的死亡，但它并不影响正常的保育细胞死亡或喂养良好的雌性的总体卵子发生。我们的数据表明半胱天冬酶在发育程序性护士细胞死亡中不起任何作用。
Cell death is a prominent feature of animal germline development. In Drosophila, the death of 15 nurse cells is linked to the development of each oocyte. In addition, females respond to poor environmental conditions by inducing egg chamber death prior to yolk uptake by the oocyte. To study these two forms of cell death, we analyzed caspase activity in the germline by expressing a transgene encoding a caspase cleavage site flanked by cyan fluorescent protein and yellow fluorescent protein. When expressed in ovaries undergoing starvation-induced apoptosis, this construct was an accurate reporter of caspase activity. However, dying nurse cells at the end of normal oogenesis showed no evidence of cytoplasmic caspase activity. Furthermore, although expression of the caspase inhibitors p35 or Drosophila inhibitor of apoptosis protein 1 blocked starvation-induced death, it did not affect normal nurse cell death or overall oogenesis in well-fed females. Our data suggest that caspases play no role in developmentally programmed nurse cell death.