Evaluation of the In vivo Radiosensitizing Activity of Etanidazole Using Tumor-bearing Chick Embryo
Evaluation of the In vivo Radiosensitizing Activity of Etanidazole Using Tumor-bearing Chick Embryo
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DOI：
10.1269/jrr.10122
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Hori, Hitoshi
中科院分区：
文献类型：
--
作者：
Abe, Chiaki;Uto, Yoshihiro;Hori, Hitoshi
荷瘤鸡胚/体内/低分化细胞放射增敏剂/依他硝唑/放射增敏活性鸡胚已被用作各种研究领域的替代实验动物，包括病毒学、免疫学、毒理学、肿瘤学和胚胎学。迄今为止，还没有使用鸡胚来评价放射增敏活性的体内模型。在这里，在体内放射增敏活性的依他硝唑，一个众所周知的乏氧细胞放射增敏剂，使用荷瘤鸡胚进行评价。根据肿瘤生长情况，对第15天鸡胚进行给药和X射线照射，终点为第18天鸡胚。在第15天的鸡胚中，等于或小于10戈伊的X射线照射剂量不引起显著的肿瘤生长抑制。静脉注射等于或小于1.0 mg的依他硝唑不会引起肿瘤生长抑制。剂量≤ 8戈伊的辐照和1.0 mg的依他硝唑均未引起鸡胚死亡。在这些结果的基础上，我们评价了8戈伊的照射和1.0 mg依他硝唑的联合治疗的放射增敏作用。如上所述，单独1.0 mg依他硝唑和单独8戈伊照射没有显示肿瘤生长抑制。相比之下，8戈伊照射和1.0 mg依他硝唑的联合治疗显示出35%的显著肿瘤生长抑制。因此，我们成功地评估了在体内的放射增敏活性的依他硝唑使用荷瘤鸡胚。这些结果表明，使用荷瘤鸡胚可能是一个有前途的系统的一部分，用于评估放射增敏活性。
Tumor-bearing chick embryo/In vivo/Hypoxic cell radiosensitizer/Etanidazole/Radiosensitizing activity.Chick embryos have been used as alternative experimental animals in various research fields, including virology, immunology, toxicology, oncology, and embryology. Until now, there have been no in vivo models using chick embryo to evaluate radiosensitizing activity. Here, the in vivo radiosensitizing activity of etanidazole, a well-known hypoxic cell radiosensitizer, was evaluated using tumor-bearing chick embryo. On the basis of tumor growth, drug administration and X-ray irradiation were performed on day 15 chick embryo, with the endpoint being day 18 chick embryo. In day 15 chick embryo, an X-ray irradiation dose of equal or less than 10 Gy did not cause significant tumor growth suppression. Intravenous administration of equal or less than 1.0 mg of etanidazole did not cause tumor growth suppression. Neither doses of equal or less than 8 Gy of irradiation nor 1.0 mg of etanidazole caused fatality of the chick embryo. On the basis of these results, we evaluated the radiosensitizing effect of a combination treatment with 8 Gy of irradiation and 1.0 mg of etanidazole. As noted above, 1.0 mg of etanidazole alone and 8 Gy of irradiation alone did not show tumor growth suppression. In contrast, a combination treatment with 8 Gy of irradiation and 1.0 mg of etanidazole showed 35% of significant tumor growth suppression. Thus, we succeeded in evaluating the in vivo radiosensitizing activity of etanidazole using tumor-bearing chick embryo. These results suggest that the use of tumor-bearing chick embryo may be part of a promising system for evaluating radiosensitizing activity.