Model-building studies of Inovirus: genetic variations on a geometric theme.
Model-building studies of Inovirus: genetic variations on a geometric theme.
复制标题
感染病毒的模型构建研究：几何主题的遗传变异。
DOI：
10.2210/pdb1ifd/pdb
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发表时间：
1990
影响因子：
8.2
通讯作者：
D. Marvin
中科院分区：
文献类型：
--
作者：
D. Marvin
Inovirus（丝状噬菌体）是一个简单的系统，用于研究蛋白质一级结构（氨基酸序列）控制二级和高级结构的规则，从而发挥功能。该病毒天然存在于许多不同的毒株中，具有相似的二级和高级结构，但组装形成病毒体外壳的蛋白质亚基在不同的病毒毒株中具有完全不同的一级结构。尽管在一级结构上存在这些差异，但所有菌株的亚基具有大致相同的大小，约50个残基，其以大致相同的方式按类型分布在一级结构的三个结构域中：N末端区域的酸性残基集合，中间附近的约19个残基的疏水结构域，以及C末端附近的碱性残基集合。每个亚基可以通过α-螺旋来近似，其长轴大致平行于纤维轴，在病毒体中从大半径到小半径倾斜，并且在下一个转弯或水平中在亚基之间交错。亚基N端附近的酸性残基在病毒体表面朝外，解释了病毒体的低等电点; C端附近的碱性残基朝内，在那里它们中和病毒体核心DNA上的电荷;疏水性中心结构域参与结合相邻亚基的相互作用。详细的X射线纤维衍射分析一个菌株给出了亚基结构。不同菌株的比较建模研究说明了共同的结构原理。
Inovirus (filamentous bacteriophage) is a simple system for studying the rules by which protein primary structure (amino acid sequence) controls secondary and higher order structure, and thereby function. The virus occurs naturally as a number of different strains with similar secondary and higher order structure, but the protein subunit that assembles to form the virion coat has quite different primary structures in different virus strains. Despite these differences in primary structure, the subunits of all strains have much the same size, about 50 residues, which are distributed by type in much the same way into three domains of primary structure: a collection of acidic residues in the N-terminal region, a hydrophobic domain of about 19 residues near the middle, and a collection of basic residues near the C-terminus. Each subunit can be closely approximated by an alpha-helix with its long axis roughly parallel to the fibre axis, sloping from large to small radius in the virion and interleaving between subunits in the next turn or level. The acidic residues near the N-terminus of the subunit face outwards on the virion surface, and explain the low isoelectric point of the virion; the basic residues near the C-terminus face inwards, where they neutralize the charge on the DNA at the core of the virion; and the hydrophobic central domain is involved in interactions which bind neighbouring subunits. Detailed X-ray fibre diffraction analysis of one strain gives the subunit structure. Comparative model-building studies of different strains illustrate the common structural principles.
丝状病毒 fd、If1、IKE、Pf1 和 Xf 的结构相似性、差异和变异性。
影响因子：
5.6
作者：
ThomasJr,GJ;Prescott,B;Day,LA
通讯作者：
Day,LA