Trypanosoma cruzi: Study of the distribution of two widespread clonal genotypes in Bolivian Triatoma infestans vectors shows a high frequency of mixed infections
Trypanosoma cruzi: Study of the distribution of two widespread clonal genotypes in Bolivian Triatoma infestans vectors shows a high frequency of mixed infections
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DOI：
10.1006/expr.1996.0075
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发表时间：
1996-08-01
影响因子：
2.1
通讯作者：
Breniere, SF
中科院分区：
文献类型：
--
作者：
Bosseno, MF;Telleria, J;Breniere, SF
两个广泛的克氏锥虫克隆基因型（20和39）的载体锥虫infestans玻利维亚标本粪便中的检测进行聚合酶链反应和克隆特异性DNA杂交的组合。对186份PCR阳性的T.在玻利维亚的两个省收集的infestans粪便中，在锥蝽属的74.2%中鉴定出克隆20，在63.4%中鉴定出克隆39。第一次，在不同地区记录了高百分比（平均值：43.2+/-26%）的混合感染（给定粪便样本中存在两个克隆）。结果与两个假设的独立传输的克隆20和39和没有选择的调查中的自然周期。对克隆20和39的地理分布的统计分析支持T。cruzi的自然克隆在不同的地方是不同的，这些差异与地方之间的距离不成比例。这些结果的流行病学意义进行了讨论。(C)北京：科学出版社. Inc.
The detection of two widespread Trypanosoma cruzi clonal genotypes (20 and 39) in feces of Bolivian specimens of the vector Triatoma infestans was performed by a combination of polymerase chain reaction and clone-specific DNA hybridization. The hybridization pattern of 186 PCR positive samples of T. infestans feces collected in two Bolivian departments identified clone 20 in 74.2% and clone 39 in 63.4% of the triatomine bugs. For the first time, a high percentage (mean: 43.2+/-26%) of mixed infections (presence of both clones in a given fecal sample) in various localities was recorded. Results were in agreement with the two assumptions of independent transmission of clones 20 and 39 and of the absence of selection in the natural cycles under survey. Statistical analysis of the geographical distribution of clones 20 and 39 favored the hypotheses that the frequencies of T. cruzi natural clones are different among localities and that these differences are not proportional to the distances that separate the localities. The epidemiological significance of these results is discussed. (C) 1996 Academic Press. Inc.