Centromeric DNA from Saccharomyces cerevisiae.
Centromeric DNA from Saccharomyces cerevisiae.
复制标题
来自酿酒酵母的着丝粒 DNA。
DOI：
10.1016/0022-2836(82)90427-2
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发表时间：
1982
影响因子：
5.6
通讯作者：
Ronald W. Davis
中科院分区：
文献类型：
--
作者：
D. Stinchcomb;Carl Mann;Ronald W. Davis
我们已经分离了29千碱基对的DNA，包含着丝粒连接基因TRP1，酿酒酵母。在分离区域内的两个DNA序列，ARS1和ars4，允许嵌合DNA分子在引入酵母后自主复制。由含有DNA分子的ars转化的酵母菌株是不稳定的；它们失去了转化的表型，同时在高频率上失去了杂交分子。另一种功能是CEN4，可以稳定含有ars的混合动力车。在有丝分裂和减数分裂过程中，CEN4允许杂交分子的适当分离。就像它的前身一样。CEN3 （Clarke & Carbon, 1980）。从基因上讲，CEN4的行为类似于酵母着丝粒。从酵母菌第4号染色体中分离到的着丝粒链序列具有非高度重复和转录活性DNA序列。我们还使用基于携带两个cenandars的分子增强有丝分裂稳定性的富集程序分离了其他cenandars。总之，ars和ceng功能允许DNA分子主要表现为独立的酵母连锁组。然而，在3 - 14%的有丝分裂细胞分裂和约3%的减数分裂分裂中，这些ear - cen杂种出现了不正确的分离，而其他酵母染色体，包括染色体IV的分离是正常的。在第一次减数分裂而不是第二次减数分裂中，不超过2%的单价圆形染色体发生姐妹染色单体的过早分离。因此，通过将着丝粒DNA片段与允许在酵母中自主复制的片段拼接在一起，可以重建功能酵母染色体。
We have isolated 29 kilobase-pairs of DNA encompassing the centromere-linked gene,TRP1, ofSaccharomyces cerevisiae. Two DNA sequences within the isolated region,ARS1 andARS4, allow autonomous replication of chimeric DNA molecules upon introduction into yeast. Yeast strains transformed byARS-containing DNA molecules are unstable; they lose the transformed phenotype and, concomitantly, the hybrid molecules at high frequency. Another function,CEN4, stabilizes theARS-containing hybrids.CEN4 allows proper segregation of hybrid molecules during mitosis and meiosis. Like its predecessor.CEN3 (Clarke & Carbon, 1980b).CEN4 behaves genetically like a yeast centromere. The centromere-linked sequences we isolated from chromosome IV of yeast are not highly repeated and contain transcriptionally active DNA sequences. We have also isolated otherCENsequences using an enrichment procedure based on the enhanced mitotic stability of molecules carrying bothCENandARS.Together, theARSandCENfunctions allow DNA molecules to predominantly behave as independent yeast linkage groups. However, improper disjunction of theseARS-CENhybrids occurs in 3 to 14% of the mitotic cell divisions and ⩽3% of the meiotic divisions while the segregation of other yeast chromosomes, including chromosome IV, is normal. No more than 2% of these monovalent circular chromosomes undergo precocious segregation of sister chromatids during the first meiotic division rather than the second. Thus functional yeast chromosomes have been reconstructed by piecing together fragments of centromeric DNA with fragments that allow autonomous replication in yeast.
影响因子：
5.6
作者：
STJOHN, TP;DAVIS, RW
通讯作者：
DAVIS, RW