A QUANTITATIVE COMPARISON OF POTENTIALLY LETHAL DAMAGE REPAIR AND THE REJOINING OF INTERPHASE CHROMOSOME BREAKS IN LOW PASSAGE NORMAL HUMAN-FIBROBLASTS
A QUANTITATIVE COMPARISON OF POTENTIALLY LETHAL DAMAGE REPAIR AND THE REJOINING OF INTERPHASE CHROMOSOME BREAKS IN LOW PASSAGE NORMAL HUMAN-FIBROBLASTS
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DOI：
10.2307/3576926
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发表时间：
1987-09-01
影响因子：
3.4
通讯作者：
BEDFORD, JS
中科院分区：
文献类型：
--
作者：
CORNFORTH, MN;BEDFORD, JS
经过长时间的放射后孵育，发现x射线暴露于非循环二倍体人成纤维细胞后过早浓缩染色体片段的残留频率与在相同处理条件下观察到的染色体畸变频率相关，当细胞传代并在有丝分裂后进行评分时。在很宽的剂量范围内，这种细胞在第一中期没有畸变的比例与能够形成宏观菌落的比例没有显著差异。此外，间期染色体断裂的重新连接率与由于潜在致命损伤（PLD）修复而增加的存活率相同。这些结果表明，G0染色体的初始断裂和重新连接与通过延迟电镀从这些细胞的平台期培养中测量的PLD的诱导和修复之间存在一对一的对应关系。
After long postirradiation incubation periods, the residual frequency of prematurely condensed chromosome fragments following X-ray exposure of noncycling diploid human fibroblasts was found to be correlated with the frequency of chromosome aberrations observed under identical treatment conditions when the cells were subcultured and scored after they reached mitosis. Over a wide range of doses, the proportion of such cells without aberrations at their first metaphase was not significantly different from the proportion able to form macroscopic colonies. Further, the rate of rejoining of interphase chromosome breaks was the same as the rate of increase in survival due to the repair of potentially lethal damage (PLD). These results suggest that there is a one-to-one correspondence between the intitial breakage and rejoining of G0 chromosomes and the induction and repair of PLD measured by delayed plating from plateau-phase cultures of these cells.